Question

Pertaining to cuvette use with a Spectrovis, identify actions that negatively affect the absorbance reading of a solution in a cuvette. Select all that apply! A. Passivating the cuvette with the solution being analyzed, then filling the cuvette with a fresh aliquot of the solution B. Wiping the clear sides of a cuvette with a Kimwipe C. Placing the cuvette in the Spectrovis so that the light passes through the frosted/ribbed sides D. Placing the cuvette in the Spectrovis so that the light passes through the clear sides E. Allowing air bubbles to remain on the sides of a cuvette during analysis of a solution

Answer 1

405- Actions that Negatively affects the Absorbance Reading of a solution in a cuvette. C. Placing the cuvette in the spectrovis so That the light passes through the frosted Ribbed sides. Justification → Cuvette is alway placed ing such a way that, the clear side is used for passing the light Frosted sides are opque,so they dont pass the light. EL. Allowing Ait bubbles to Remain on the sides of a cuvette during Analysis. of a solution. Justification Aik bubbles in cuvette may cause Fluctuation in Reading