Upon performing a nitrate test, you observe the following: No gas in the Durham tube, sediment,...
Upon performing a nitrate test, you observe the following: No gas in the Durham tube, sediment, and after addition of the nitrate reagents A & B, the media remained clear/yellow. The next step would be to add ______ to the tube.
| A. | Bacteria | |
| B. | Oxidase reagent | |
| C. | Zinc | |
| D. | Kovacs reagent |
Homework Answers
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Upon performing a nitrate test, you observe the following: No
gas in the Durham tube, sediment,...
Nitrate reduction test 3 Suppose yout has a gas bubble in the Durham tube. Knowing that...
Nitrate reduction test
3 Suppose yout has a gas bubble in the Durham tube. Knowing that fermenters frequenthy produce gas, you ignore the bubl and proced to the next step. Adding reagents produces no chang consistent with what you have learned about this test? Why? remove your test cultures from the incubator and notice that one of them-a known fermenter- e, and neither does addng zinc. Is this occurrence 4 Would you change your answer to question 3 if the...
?? Which of the following would be true for a negative nitrate reduction test? The nitrate...
?? Which of the following would be true for a negative nitrate reduction test? The nitrate broth remains clear after the addition of both reagents A and B and then zinc The nitrate broth turns red after the addition of reagents A and B. The nitrate broth remains clear after the addition of reagents A and B but turns red after the addition of zinc. The nitrate broth turns red before the addition of the reagents A and B and...
bubble after incubation. b. There is no gas (or you know the gas is from fermentation)...
bubble after incubation. b. There is no gas (or you know the gas is from fermentation) and the tube turns red after the addition of reagents A and B. c. There is no color after the addition of A and B and after the addition of zinc powder. Questions 1. Would expect Clostridium tetani to possess the enzyme catalase? Explain. 2. Why does the Nitrate reduction tube turn red after the addition of zinc? 3. What role does cytochrome c...
bubble after incubation. b. There is no gas (or you know the gas is from fermentation)...
bubble after incubation. b. There is no gas (or you know the gas is from fermentation) and the tube turns red after the addition of reagents A and B. There is no color after the addition of A and B and after the addition of zinc powder. Questions 1. Would expect Clostridium tetani to possess the enzyme catalase? Explain. 2. Why does the Nitrate reduction tube turn red after the addition of zinc? 3. What role does cytochrome c oxidase...
You have obtained samples from a patient who is in the ICU at your hospital. You...
You have obtained samples from a patient who is in the ICU at your hospital. You perform the Gram-Stain and IMVIC tests, and see the following results: Gram-Stain Upon examination under the microscope you view pink colored rods. IMVIC Indole: After the addition of Kovac's Reagent, you see no color change at the top of the media. Methyl Red: After the addition of methyl red, your broth immediately turns a red color. Voges Proskauer: After the addition of VP reagents...
help please! 81% Help % Lab 39 worksheet 25% + Room Add Page Insert Durham Tube...
help please!
81% Help % Lab 39 worksheet 25% + Room Add Page Insert Durham Tube Sugar Fermentations Table Chart Text Shape Media Comment 1. Which tube, red or yellow, is positive for sugar fermentation? 2. Is the color change an increase or decrease in pH? 3. Which two gases can be revealed by the bubble in the inverted tube? Mixed Acid (MR test) and Butanediol production (Voges:Proskauer, VP test) 1. Which group of bacteria is the Mixed Acid test...
With these results which bacteria can it be? Alcaligenes faecalis Enterobacter aerogenes Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Gram Stain Results Organism Cell...
With these results which bacteria can it be?
Alcaligenes faecalis
Enterobacter aerogenes
Escherichia coli
Proteus vulgaris
Pseudomonas aeruginosa
Serratia marcescens
Gram Stain Results Organism Cell Shape Gram Stain Cell Arrangement Single Unknown sample Gram negative Rod Oxidase Results Color after Adding Reagent Organism Unknown sample Yellow, Negative SIM Results Motility Organismm Sulfur reductionn Indole production Unknown sample EMB Results Organismm Unknown sample Growth Colony Appearance Raised, Circular, Entired Purple, some colonies Glucose and Lactose Fermentation Glucose Lactose Organism Growth Color...
Test interpretation: A. Addition of reagents A and B: color changed to redà nitrate reductase- positive...
Test interpretation:
A. Addition of reagents A and B: color changed to redà nitrate
reductase- positive (nitrate reducer). If this is the case, you are
done, do not add zinc powder.
B. Addition of reagents A and B: no color changeà it could be:
(a) nitrate reductase-negative (non-reducer) or (b) nitrate
reductase-positive (super reducer). ADD ZINC POWDER TO THE BROTH to
distinguish between these two possibilities.
1. If red color formsà nitrate is still present in the broth:
non- reducer....
C. Zn + H2SO, d. Zn + PbCl2 2. When ammonium carbonate (NH.COs decomposes, what gas...
C. Zn + H2SO, d. Zn + PbCl2 2. When ammonium carbonate (NH.COs decomposes, what gas is produced along with NH? Would a glowing splint burn brighter in the presence of this gas? Explain your answer 3. Your teacher hands you a test tube containing a clear solution with either dissolved Na CO, or dissolved NaCl. Considering the double-replacement reactions you performed, what one reagent would you add to determine your test tube contents? Describe what you would expect to...
find the unknown bacteria based on this results? List of possible Bacteria • Selective Unknown Bacteria...
find the unknown bacteria based on this results?
List of possible Bacteria • Selective Unknown Bacteria # 4. and Differential Media results for 4 Test MSA PEA Gram (+) to Bacillus cereus (bacillus) do 2. Bacillus mepeterum (becillus) 3. bacillus subtilis (bacillus) Blood 1-Starch Results Interpretation Presumptive ID red frowth Orpinism does not ferment manitol. Nonpathogenic No reochrom (-) good growth oganism is not inhibitelje gram Phenylethyl alcohol complete olipeskon 14 red blood cells on o nomeync activity B hemolytic...
Nitrate reduction test
3 Suppose yout has a gas bubble in the Durham tube. Knowing that fermenters frequenthy produce gas, you ignore the bubl and proced to the next step. Adding reagents produces no chang consistent with what you have learned about this test? Why? remove your test cultures from the incubator and notice that one of them-a known fermenter- e, and neither does addng zinc. Is this occurrence 4 Would you change your answer to question 3 if the...
?? Which of the following would be true for a negative nitrate reduction test? The nitrate broth remains clear after the addition of both reagents A and B and then zinc The nitrate broth turns red after the addition of reagents A and B. The nitrate broth remains clear after the addition of reagents A and B but turns red after the addition of zinc. The nitrate broth turns red before the addition of the reagents A and B and...
bubble after incubation. b. There is no gas (or you know the gas is from fermentation) and the tube turns red after the addition of reagents A and B. c. There is no color after the addition of A and B and after the addition of zinc powder. Questions 1. Would expect Clostridium tetani to possess the enzyme catalase? Explain. 2. Why does the Nitrate reduction tube turn red after the addition of zinc? 3. What role does cytochrome c...
bubble after incubation. b. There is no gas (or you know the gas is from fermentation) and the tube turns red after the addition of reagents A and B. There is no color after the addition of A and B and after the addition of zinc powder. Questions 1. Would expect Clostridium tetani to possess the enzyme catalase? Explain. 2. Why does the Nitrate reduction tube turn red after the addition of zinc? 3. What role does cytochrome c oxidase...
You have obtained samples from a patient who is in the ICU at your hospital. You perform the Gram-Stain and IMVIC tests, and see the following results: Gram-Stain Upon examination under the microscope you view pink colored rods. IMVIC Indole: After the addition of Kovac's Reagent, you see no color change at the top of the media. Methyl Red: After the addition of methyl red, your broth immediately turns a red color. Voges Proskauer: After the addition of VP reagents...
help please!
81% Help % Lab 39 worksheet 25% + Room Add Page Insert Durham Tube Sugar Fermentations Table Chart Text Shape Media Comment 1. Which tube, red or yellow, is positive for sugar fermentation? 2. Is the color change an increase or decrease in pH? 3. Which two gases can be revealed by the bubble in the inverted tube? Mixed Acid (MR test) and Butanediol production (Voges:Proskauer, VP test) 1. Which group of bacteria is the Mixed Acid test...
With these results which bacteria can it be?
Alcaligenes faecalis
Enterobacter aerogenes
Escherichia coli
Proteus vulgaris
Pseudomonas aeruginosa
Serratia marcescens
Gram Stain Results Organism Cell Shape Gram Stain Cell Arrangement Single Unknown sample Gram negative Rod Oxidase Results Color after Adding Reagent Organism Unknown sample Yellow, Negative SIM Results Motility Organismm Sulfur reductionn Indole production Unknown sample EMB Results Organismm Unknown sample Growth Colony Appearance Raised, Circular, Entired Purple, some colonies Glucose and Lactose Fermentation Glucose Lactose Organism Growth Color...
Test interpretation:
A. Addition of reagents A and B: color changed to redà nitrate
reductase- positive (nitrate reducer). If this is the case, you are
done, do not add zinc powder.
B. Addition of reagents A and B: no color changeà it could be:
(a) nitrate reductase-negative (non-reducer) or (b) nitrate
reductase-positive (super reducer). ADD ZINC POWDER TO THE BROTH to
distinguish between these two possibilities.
1. If red color formsà nitrate is still present in the broth:
non- reducer....
C. Zn + H2SO, d. Zn + PbCl2 2. When ammonium carbonate (NH.COs decomposes, what gas is produced along with NH? Would a glowing splint burn brighter in the presence of this gas? Explain your answer 3. Your teacher hands you a test tube containing a clear solution with either dissolved Na CO, or dissolved NaCl. Considering the double-replacement reactions you performed, what one reagent would you add to determine your test tube contents? Describe what you would expect to...
find the unknown bacteria based on this results?
List of possible Bacteria • Selective Unknown Bacteria # 4. and Differential Media results for 4 Test MSA PEA Gram (+) to Bacillus cereus (bacillus) do 2. Bacillus mepeterum (becillus) 3. bacillus subtilis (bacillus) Blood 1-Starch Results Interpretation Presumptive ID red frowth Orpinism does not ferment manitol. Nonpathogenic No reochrom (-) good growth oganism is not inhibitelje gram Phenylethyl alcohol complete olipeskon 14 red blood cells on o nomeync activity B hemolytic...
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