Question

Test interpretation:

A. Addition of reagents A and B: color changed to redà nitrate reductase- positive (nitrate reducer). If this is the case, you are done, do not add zinc powder.

B. Addition of reagents A and B: no color changeà it could be: (a) nitrate reductase-negative (non-reducer) or (b) nitrate reductase-positive (super reducer). ADD ZINC POWDER TO THE BROTH to distinguish between these two possibilities.

1. If red color formsà nitrate is still present in the broth: non- reducer.

2. No red color forms à no nitrate present, because it was converted to nitric oxide, nitrous oxide, ammonia or nitrogen gas: super nitrate reducer.

Write positive or negative in the categories of the table above for each Nitrate broth tube.

Tube # Nitrate reductase Nitrite reductase Super reducer A A B C Click or tap Click or tap here to enter here to enter text. text. Click or tap here to enter text. B Click or tap Click or tap here to enter here to enter text. text. Click or tap here to enter text. C Click or tap Click or tap here to enter here to enter text. text. Click or tap here to enter text.

Answer 1

After including 1 dropper of sufanilic acid as well as one dropper of an alpha naphthylamine to each and every broth,a colour shift to RED signifies a good nitrate reduction check as revealed around examination tube A, will mean organism has decreased nitrate to nitrite.

No colour shift suggest lack of nitrite as revealed in test tube B.

Test tube C is actually leading nitrite diminished directly into nitrogen dioxide (NO2), test result that is positive of nitrite reduction test