Question

bubble after incubation. b. There is no gas (or you know the gas is from fermentation) and the tube turns red after the addition of reagents A and B. c. There is no color after the addition of A and B and after the addition of zinc powder. Questions 1. Would expect Clostridium tetani to possess the enzyme catalase? Explain. 2. Why does the Nitrate reduction tube turn red after the addition of zinc? 3. What role does cytochrome c oxidase play in aerobic respiration?

Answer 1

cytochrome c oxidase (COX) is the terminal complex of the electron transfer chain.This enzyme of the inner mitochondrial membrane catalyzes the last step of respiration, It accepts electrons from reduced Cytc and transfers them to molecular oxygen, which is reduced to water. Hence it plays a vital role in energy production of aerobic cells.

2) Answer:- Clostridium tetani is a rod shaped gram positive bacteria. It is strictly unaerobic in nature. It is Sensitive to heat & cannot Survive in the presence of Oxygen. Hence, it would not possess catalase enzyme, because catalase enzyme found in organisms exposed to Oxygen. It catalyzes the decomposition of hydrogen peroxide to water & Oxygen. 21 Nitrate reduction tube turn red after the addition of Linc, as Zinc powder catalyses the reduction of nitrate to nitrite. A red color is produced in the medium only cohen nibrite is present in the medium, otherwise it remains as itis, c colorless). When Pal Zinc is added to tube, it catalyses the gecluchior Od nitrate to nitrite, which then reacts with Nitrate reagen Suifanic acid & alpha-naphy - -thylamine to give red color. The red 0 Color suggest that nitrate was not reduced by organism and the test organismo isleben not capable of reducing nitrate.