Question

61. How many different test results can you get from 1 SIM test? a. 1 b. 2 c. 3 d. 4 62. How can SIM medium be used to assess motility? a. Growth radiates outward in all directions from the stab line and on the surface b. Media turns black c. Formation of a pink color on the surface of the agar d. More than 1 of the above 63. What type of respiration results in the production of H2S during the SIM medium test (see Ch of the textbook too)? a. Aerobic respiration b. Anaerobic respiration c. Fermentation d. None of the above 64. How would you know if the microbe produces either enzyme cysteine desulfurase or thiosulfa reductase? a. The media turns green b. A gas bubble pushes the media off the bottom of the test tube c. The media turns black d. The media liquifies 65. What enzyme produces the diagnostically important product indole from the amino acid trypto a. Indole dehydrogenase b. Tryptophanase c. Tryptophan decarboxylase d. More than 1 of the above 66. Which of the following is necessary to test for indole production? a. Formation of a pink color in the surface of the agar. b. Media turns black. c. Formation of a pink color after adding Kovac's reagent. d. More than 1 of the above 67. Which of the following is resistant to the antibiotic Bacitracin (Lab Exercise 5-20)? a. Micrococcus b. Staphylococcus c. Streptomyces d. Bacillus

Answer 1

61) c-3: SIM test can be used identify 3 test -sulfur production,motility, indole production.

62)a Growth radiates in all direction outward from the region os stab. A positive motility test is indicated by a diffuse zone of growth flaring from the line of inoculation.

63)b aearobes an sulfur reducing bacteria

64)c- the media turns black in the presence of H2S.

65)b- typtophanase breaks down trptophan and produce indol.

66)c-indole is detected by production of pink colour by adding kovacs reagent.

67)d- Bacillus, Bacitracin is an antibiotic produced by certain species of Bacillus