Solubility of DNA in various solutions
DNA solubility in:
Water
Isotonic (0.15M) salt solution
2.5 M saline
Since DNA is polar due to its highly charged phosphate backbone. DNA is soluble in water, Isotonic(0.15M) salt solution and 2.5M saline.
Solubility of DNA in various solutions DNA solubility in: Water Isotonic (0.15M) salt solution 2.5 M...
7. Which of the following solutions is isotonic (no osmosis) with a 0.30 M NaNO, solution? {hint - Find the osmosis molarity or "osmolarity" = (# ions)M} a) 0.10 M CaCl2 b) 0.30 M glucose c) 0.60 M NaCl d) 0.15 M AICI; e) 0.75 M KCI (n-1 for glucose) 8. If a solution of 0.20 M Mg(NO3)2 is separated from a 0.25 M KCl solution by a membrane that is permeable to water, osmosis will occur. Describe the osmotic...
In osmosis, water flows from hypertonic/hypotonic/isotonic (highlight one) solutions to hypertonic/hypotonic/isotonic (highlight one) solutions, until both solutions are hypertonic/hypotonic/isotonic (highlight one).
I just recently conducted an experiment in my biochemistry class where we had to add DNA to distilled water, isotonic saline and 2.5M NaCl. I noticed different physical aspects depending on the solvent, but I'm not sure how I would categorize them. Here's what I saw: Distilled: fibrous precipitate, cloudy liquid Isotonic: Honestly barely noticed a change here? Maybe I wasn't looking hard enough 2.5M: salt precipitate at the bottom and fibres I know DNA is polar so solubility should...
Calculating the Effect of a Common Ion on Solubility Consider a saturated solution of the salt MX3, which M is a metal cation with a 3+ charge and X is an anion with a 1− charge, in water at 298 K. Which of the following will affect the Ksp of MX3 in water? Consider a saturated solution of the salt , which is a metal cation with a charge and is an anion with a charge, in water at 298 . Which of the...
Salt solutions are generally more dense than pure water samples. The density of the calcium hydroxide solution was assumed to be that of water. Considering this error, the recorded volume of calcium hydroxide solution is greater than / less than the actual. The recorded value of solubility (S) is greater than / less than the actual. The recorded value of Ksp is greater than / less than the actual.
Need a help here please Determine if the following solutions are isotonic (0.28M in solutes), hypotonic, or hypertonic solution containing 0.28 M NaCl isotonic solution containing 0.28 M glucose isotonic solution containing 0.1 M magnesium nitrate [Mg(NO3)2] hypotonic solution containing 0.05 M KNO3 and 0.23 M glucose isotonic solution containing 0.1 M NaCl and 0.01 M CaCI2 hypotonic
Table 1 Salt Solution Preparations Salt Solutions* Volume of Solution Calculated Mass of Solid Salt Baking soda (0.1 M NaHCO3) 10 mL Fritz PRO (0.1M NH4CI) 10 mL Note: *Use the Molarity equation (M (grams) needed to prepare the salt solution. moles/liter solution) to calculate the amount
2. If a person cannot drink salt water, why can they drink sugar water? 3. Carrots were weighed, soaked in 4 different salt solutions and then re-weighed. Horace collected the weight changes (g) carefully: +2, -4,-1,0 Unfortunately, he forgot which weight change matched which salt solution. Predict which numbers should go where on the table below. Salt (g) added to 300 ml Weight change (g) water 10 20 5. In the table above, when the weight change is "O", the...
Show calculations for the solutions. Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
Calculate the percent saline (NaCl) solution that would be isotonic. The molecular weight of NaCl is 58.44 g/mol. Show your calculations. (Hint #1: start with normal cell and plasma osmolality, which is 0.3 Om/kg and convert that to percent saline. Hint #2: NaCl is ionic, so it dissociates in water).