Question

2. Enzymes that we are testing for would be produced by the that is being catabolized is considered a while the amino acid material or ingredient. 3. Indicate, with arrows, where each of the following enzymes breaks a bond in the amino acid. Phenylalanine Deaminase Lysine Decarboxylase Tryptophanase Cysteine Desulfhydrase 4. Kovac's reagent is added to the medium and will form a ring at the top of the media if the result is positive. The enzyme that may be produced (or not) by the bacteria is The end product is called _. A positive result indicates a potential S. FeCl, is added to the medium and will turn a result is positive. A positive result indicated the enzyme produced by the bacteria. color if the - was 6. The pH indicator in the lysine test is . A positive lysine test will be a color. If the result is purple, what enzyme did the bacteria produce? 7. The pH indicator in the urease medium is and a positive result will be a _color. A positive urease test indicated a potential 8. SIM stands for and a. Describe what a positive test for sulfur would look like: b. What enzymes, produced by bacteria, can create a positive Stest? c. List two things have your learned about a species that is positive for sulfur d. A positive indole test would have a at the top of the tube. e. What enzyme, produced by the bacteria, would result in a positive indole reaction? f. What is the differential ingredient for the indole test?

Answer 1

2. Produced by bacteria; media material or ingredient

3. Phenylalanine deaminase - Red arrow

Lysine decarboxylase - Green arrow

Tryptophanase - Blue arrow

Cysteine desulfhydrase - Yellow arrow

4. Culture medium; red ring; tryptophanase enzyme; end product indole; potential E. coli.

5. Culture medium; purple color; phenylalanine deaminase

6. Bromocresol purple, cresol red; red ; lysine decarboxylase

7. Phenol red; Pink; Proteus bacteria

8. Sulfide, Indole, Motility