Following the addition of naphthylamine to a tube of nitrate broth culture, no color change occurs....
Following the addition of naphthylamine to a tube of nitrate broth culture, no color change occurs. Zinc dust is then added, and again no color change occurs. A gas bubble is observed in the Durham tube. These results indicate that:
| more testing is required. |
| denitrification has occured. |
| nitrate has been reduced to nitrite. |
| nitrate has likely been converted to ammonia. |
| nitrate has not been utilized. |
Homework Answers
These results indicate that-
Nitrate has been likely converted to ammonia ,as a gas bubble is observed in the inverted Durham tube as zinc dust is added and no colour change occured.
Add Answer to:
Following the addition of naphthylamine to a tube of nitrate
broth culture, no color change occurs....
Test interpretation: A. Addition of reagents A and B: color changed to redà nitrate reductase- positive...
Test interpretation:
A. Addition of reagents A and B: color changed to redà nitrate
reductase- positive (nitrate reducer). If this is the case, you are
done, do not add zinc powder.
B. Addition of reagents A and B: no color changeà it could be:
(a) nitrate reductase-negative (non-reducer) or (b) nitrate
reductase-positive (super reducer). ADD ZINC POWDER TO THE BROTH to
distinguish between these two possibilities.
1. If red color formsà nitrate is still present in the broth:
non- reducer....
Nitrate reduction test 3 Suppose yout has a gas bubble in the Durham tube. Knowing that...
Nitrate reduction test
3 Suppose yout has a gas bubble in the Durham tube. Knowing that fermenters frequenthy produce gas, you ignore the bubl and proced to the next step. Adding reagents produces no chang consistent with what you have learned about this test? Why? remove your test cultures from the incubator and notice that one of them-a known fermenter- e, and neither does addng zinc. Is this occurrence 4 Would you change your answer to question 3 if the...
Questions: After adding Nitrate Reagent A and B, there was no red color change. You then...
Questions: After adding Nitrate Reagent A and B, there was no red color change. You then added a pinch of zinc powder with a formation of a red color. What category of nitrate reduction, would this microbe be? Explain how this red color change occurred? Denitrification part of the nitrogen cycle. Some microbes use this pathway anaerobically to convert nitrates (NO3- ) to nitrogen (N2) gas. Other bacteria in anaerobic conditions, use what is called a Dissimilatory nitrate reduction to...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B...
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B by-product of microbial metabolic activities C icorial growth D host-preste relationship E None of the above 2.Microbes muy compete ayust the environment tempera requirements in order to compete for nutrients A True B. False , c.) to the prima A B The pathogenesis of disease can move son of issues by extracellule enzymes or physical blocking of capillaries due to unlimited microbial greneth True...
Test interpretation:
A. Addition of reagents A and B: color changed to redà nitrate
reductase- positive (nitrate reducer). If this is the case, you are
done, do not add zinc powder.
B. Addition of reagents A and B: no color changeà it could be:
(a) nitrate reductase-negative (non-reducer) or (b) nitrate
reductase-positive (super reducer). ADD ZINC POWDER TO THE BROTH to
distinguish between these two possibilities.
1. If red color formsà nitrate is still present in the broth:
non- reducer....
Nitrate reduction test
3 Suppose yout has a gas bubble in the Durham tube. Knowing that fermenters frequenthy produce gas, you ignore the bubl and proced to the next step. Adding reagents produces no chang consistent with what you have learned about this test? Why? remove your test cultures from the incubator and notice that one of them-a known fermenter- e, and neither does addng zinc. Is this occurrence 4 Would you change your answer to question 3 if the...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
LAR STUDY GUM Exercises 76, 77, 78, 79,719, 7.11.8.19.2.0.7.0 1. Define niche A ecological envi B by-product of microbial metabolic activities C icorial growth D host-preste relationship E None of the above 2.Microbes muy compete ayust the environment tempera requirements in order to compete for nutrients A True B. False , c.) to the prima A B The pathogenesis of disease can move son of issues by extracellule enzymes or physical blocking of capillaries due to unlimited microbial greneth True...
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