ZuoTi.Pro
Question

Genetics Lab Part A: If an alignment has a very low E value, does this mean...

Genetics Lab

Part A:

If an alignment has a very low E value, does this mean that the two sequences are homologous? IF the answer is "No", then what does a very low E value mean?

Define Homologous as it is used when talking about DNA sequences.

How low an E value do you need to infer that the two sequences that are aligned are similar biologically?

What does an E Value of 0.0 mean as far as 1e-?? is concerned? What does it mean as far as sequence similarity is concerned?

science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

If a sequence has low value this means that this is the best hit which means they have more similarity. The lower the E value, the better it is. Homologous DNA sequence are those which share similarity in nucleotide sequence. Smallest e value that can be considered is 0.01. A value greater than 0 and smaller than 1 is considered An e value of 0.0 means that there is no sequence available that will match with query sequence

0 0
Add a comment
Know the answer?
Add Answer to:
Genetics Lab Part A: If an alignment has a very low E value, does this mean...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • E-value for microbial computational genomics/genetics? Particularly need help with 1a, but if you can help with...

    E-value for microbial computational genomics/genetics? Particularly need help with 1a, but if you can help with any of the rest that would be great! Thanks in advance! 1. As discussed in the final computational lecture, PSI-BLAST' is an iterative multiple sequence alignment technique that starts with a single protein query of database search by pairwise alignment. The resulting pairwise alignments from that search are collated and analyzed to create a PSSM (position specific substitution matrix) which is subsequently used in...

  • 5. Professor Clueless has just identified a novel sequence X that s VERY similar to sequence Y as shown by the alignment in Figure 16.2. On the other hand, when he uses blast to find homologs of X ag...

    5. Professor Clueless has just identified a novel sequence X that s VERY similar to sequence Y as shown by the alignment in Figure 16.2. On the other hand, when he uses blast to find homologs of X against genbank (Figure 16.3), he obtains VERY different results from a blastn with query sequence Y (Figure 16.4). With such similarity between X and Y, he was expecting the two queries to get very similar results. What advice will you give the...

  • The E-value is a measure of how significant the similarity is between two sequences. The lower...

    The E-value is a measure of how significant the similarity is between two sequences. The lower the E value, the more significant the match (i.e., the more confident you should be that the sequence BLAST found is a biologically interesting match). (A reminder of the shorthand used in the BLAST results page: an E-value of 8e-21 is 8 times 10^-21 in scientific notation.) (a) According to the YouTube video, what is the maximum E value score that could still be...

  • A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between...

    A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between genes play an important role in assigning gene function? Successful insertion of a DNA fragment into the multi-cloning region (restriction sites) of a recombinant plasmid is detected by what changes? Understand the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis. In addition to restriction enzymes, which enzyme(s) are required to insert a fragment of DNA into a cloning vector? What is...

  • What is the Central Dogma of genetics? -- EXPLAIN Corynebacterium diptheriae, the causative agent of diptheria,...

    What is the Central Dogma of genetics? -- EXPLAIN Corynebacterium diptheriae, the causative agent of diptheria, secretes a toxin that enzymatically inactivates all molecules of elongation factor in a eukaryotic cell. What immediate and long-term effects does this have on the cellular metabolism of an infected person? -- EXPLAIN What is the difference between horizontal gene transfer and vertical gene transfer? -- EXPLAIN After running gel electrophoresis, what do the black bands represent? What can those bands tell us, and...

  • help! Post-Lab Questions 1. Does a large value for Rf mean that the solute has a...

    help! Post-Lab Questions 1. Does a large value for Rf mean that the solute has a relatively strong affinity for the solvent (mobile phase) or a relatively weak affinity for the solvent? 2. Briefly describe what experimental conditions gave you the best separation. 3. The structure of indigo is shown in the discussion section. This type of structure is common for organic molecules and not all atoms, lone pairs and bonds are shown. Use your textbook as mentioned earlier in...

  • LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...

    LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...

  • SOLVE ONLY PART E has mean that has changecl tom 40 Assume that, when 1. There is a concern a voltage generator tested, the output χ will have mean μ with standard deviation σ2) HoY 40 vs. Ha:μ#40 a...

    SOLVE ONLY PART E has mean that has changecl tom 40 Assume that, when 1. There is a concern a voltage generator tested, the output χ will have mean μ with standard deviation σ2) HoY 40 vs. Ha:μ#40 a) (3pts) What kinds of values of 3 would lead to the rejection of the null hypothesis? In other words, what is the correct form of the rejection region (RR) ? SELECT A,B,C,D,E, or F: Answer A) Significantly larger than 40 B)...

  • 2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...

    2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...

  • Part I— Just Bad Luck? Brrrring! Brrrring! Jane checked the caller ID on her phone. “Sam!...

    Part I— Just Bad Luck? Brrrring! Brrrring! Jane checked the caller ID on her phone. “Sam! Great!” she thought. It was always nice to get a call from her older brother. But a little twinge of worry tugged at her. It was just a couple of weeks ago that he had mentioned making an appointment with his doctor about some abdominal pain he had been having. “Hi Sam! It’s great to hear from you,” Jane answered. “Hi Jane. Well I...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT