Question

PD, NPD ou T PD 21 NPD 5 FEEEEEEEEEEEEEEEEE Grey color gen Tan color gene #rec Genotype #non rec #non #rec spores Total spores rec. spores spores gut gut gt+ gt+ 104 0 104 13 gt+ gt+g+tg+t 152 19 g+t+g+t+ gt gt gt gt g+t+g+t+ - 40 gt gut g+t+g+ 9 gut gt gt+ g+t+ 11 gut gt g+t+g+ 9 g++ g+t gt+ gt 15 gt+ gut g+t+ gt 2 g+t+ gt gt+g+t g+t+ gt g+t+gt g+t+ gut gt ge+ gt+ gtg+t ge+ g+t+ gt gt g+t+ g+t+ gt gut ge+ g+tg+t+gt+ gt gt g+t+g+t+ gt gut gt+ gt+g+t gut gt+g+t+ gt g+t g+t+ gt gt+ gt g+t+g+t g+ 11 gut gt+ gt g+t+ 5 gut gt+ gut gt+ 4 4 4 4 i Total 436 1156 448 1144 1 99 7 13

This dataset was obtained from a group of students who completed lab 4. What are (1) the OBSERVED FREQUENCY OF NON RECOMBINANT ASCI (first submission box) and (2) the PREDICTED FREQUENCY OF NON RECOMBINANT ASCI (second submission box)? Your values should be rounded to the nearest second digit after the decimal point. Use a dot to display the decimal point, not a comma. You should assume that the true genetic distance for the gray allele is 33 cM and for the tan allele 27 cM. Also remember that a frequency value should be between 0 and 1.

Answer 1

Total number Of Ascus - 3184

Number of non recombinant Ascus - 2300

So, Observed freequency of Non - Recombinant Ascus - 2300/3184 = 0.72.

Now theoritically speaking PD are 100% non remombinant

NPD are 100% recombinant and T are 50% recobinant and 50% non recombinant.

So, Here all the PD are predicted to be non recombinant- 312

and 50% of T types - 50% of 1372 = 686

So, total number of non recombinant ascus = 998

So, Predicted freequency of Non - Recombinant Ascus - 998/3184 = 0.31.

For better Understanding , an example with different gene names,

Haploid cell uratarg x ura-2arg-3 Haploid cell ura ura-2 arg'arg-3 Diploid zygote Meiosis Possible asci: uraarg ura'arg ura'arg-3 ura-2arg-3 uratarg-3 ura-2arg ura-2arg-3 ura-2arg-3 ura-2arg ura'arg ura-2arg uratarg-3 Parental ditype 2 ura arg : 2 ura-2arg-3 Tetratype 1 uraarg: 1 ura-2arg : 1 ura'arg-3: 1 ura-2arg-3 Nonparental ditype 2 ura'arg-3: 2 ura-2arg PD ascus: contains 100% parental cells T ascus: contains 50% parental cells and 50% recombinant cells NPD ascus: contains 100% recombinant cells