Question

What is the function of the protein trypsin in removing adherent cells from a plate?

Group of answer choices

A. It digests the specific cellular proteins that are involved in adhesion of the cells to the plate.

B. It interferes with the binding of the cells to the plate.

C. It digests the serum in the medium, hence making the cells come off the plate.

D. It changes the pH of the medium, making the cells come off the plate.

Answer 1

Answer:  It digests the specific cellular proteins that are involved in the adhesion of the cells to the plate.

Explanation: Trypsin which is a proteolytic enzyme that is used to breakdown the proteins to detach adherent cells from the plate or vessel. It segments the lysine or arginine which is present on the C terminal side of peptide chain. EDTA which is a metal chelator act as a supplement to trypsin solution which increases the activity. The action of EDTA is to detach the calcium and magnesium from the surface of cell. By this action of the EDTA, the hydrolyzation is performed for definite or specific peptide bonds.