Homework Answers
Lands lysogen means the chromosome of the bacteria have also integrated genome of lands phage which is called prophages.
Wild type lands phage can infect but in lysogenic life cycle one virus Donny kill the bacteria rather integrate their genome with bacterial genome and make many copies as the total genome and then it can enter the lytic phage and kill the bacteria and can produce many form but here, in lysogenic form they can't kill the bacteria .
Thank you
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5 points. Can a wild-type lambda phage infect and kill an E. coli host that harbors...
5 points. When lambda infects an E. coli host what are the first two proteins that...
5 points. When lambda infects an E. coli host what are the first two proteins that are produced? 5 points. In a lambda lysogen, what proteins are produced?
synergism Page 2 of 5 Rabibove love 18. The Spectrum of cells a virus can infect...
synergism
Page 2 of 5 Rabibove love 18. The Spectrum of cells a virus can infect is defined as the host mange. Which of the following viral pathogens has the widest host range? & Hepatitis B adsorbs to human liver calls & Poliovirus attaches to intestinal and nerve cells c Rabies attaches to several cells of many mammals a all the above C none of the above 19. In Persistent infections, the cell harbors the virus, which is not immediately...
Question 2 (14 pts) Position 210 in the wild-type, tryptophan synthetase A protein of E. coli...
Question 2 (14 pts) Position 210 in the wild-type, tryptophan synthetase A protein of E. coli is occupied by glycine. The following sequences of amino acid substitutions and restitutions occur at this position: ile ----->arg<----->gly<--->glu A. Show the codons and the one-letter alterations that will account for the changes shown in the amino acids. (A codon chart is provided.) B. Which of the amino acid changes are the results of transitions? Which are the results of transversions? C. Give one...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm Tet' gene pxyls HOC 5 G. E. coli Amp' gene Kan gene Mutants of this transformed E. coli strain can be obtained by exposing the cells to dimethyl sulfate. Briefly, explain how you might screen these mutants to obtain an xyls variant that can bind to 4-ethylbenzoic acid 1, carefully explaining the logic underlying your protocol. You can assume that wild type xyls cannot...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
1. The 1952 experiment performed by the Lederbergs in which they replica-plated ten million colonies of...
1. The 1952 experiment performed by the Lederbergs in which they replica-plated ten million colonies of TI-phage sensitive E. coli to plates containing high concentrations of TI phase and found a small number of phage-resistant colonies showed that: A) a selecting agent can affect mutation rate in E. coli. B) mutations can arise spontaneously before exposure to the selecting agent. C) mutations in E. coli occur at a relatively high frequency, D) the mutation rate in E. coli fluctuates greatly...
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1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
As a student project, you have isolated six new mutant strains of E. coli with altered...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
5 points. When lambda infects an E. coli host what are the first two proteins that are produced? 5 points. In a lambda lysogen, what proteins are produced?
synergism
Page 2 of 5 Rabibove love 18. The Spectrum of cells a virus can infect is defined as the host mange. Which of the following viral pathogens has the widest host range? & Hepatitis B adsorbs to human liver calls & Poliovirus attaches to intestinal and nerve cells c Rabies attaches to several cells of many mammals a all the above C none of the above 19. In Persistent infections, the cell harbors the virus, which is not immediately...
Question 2 (14 pts) Position 210 in the wild-type, tryptophan synthetase A protein of E. coli is occupied by glycine. The following sequences of amino acid substitutions and restitutions occur at this position: ile ----->arg<----->gly<--->glu A. Show the codons and the one-letter alterations that will account for the changes shown in the amino acids. (A codon chart is provided.) B. Which of the amino acid changes are the results of transitions? Which are the results of transversions? C. Give one...
(c) An E. coli strain was created by transforming with the two plasmids shown below: pm Tet' gene pxyls HOC 5 G. E. coli Amp' gene Kan gene Mutants of this transformed E. coli strain can be obtained by exposing the cells to dimethyl sulfate. Briefly, explain how you might screen these mutants to obtain an xyls variant that can bind to 4-ethylbenzoic acid 1, carefully explaining the logic underlying your protocol. You can assume that wild type xyls cannot...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
1. The 1952 experiment performed by the Lederbergs in which they replica-plated ten million colonies of TI-phage sensitive E. coli to plates containing high concentrations of TI phase and found a small number of phage-resistant colonies showed that: A) a selecting agent can affect mutation rate in E. coli. B) mutations can arise spontaneously before exposure to the selecting agent. C) mutations in E. coli occur at a relatively high frequency, D) the mutation rate in E. coli fluctuates greatly...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
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