can someone explain PEP-PTS ( Phosphoenolpyruvate phosphotransferase system)
In metabolism, which will come first: Dephosphorylation of phosphoenolpyruvate (PEP) or dephosphorylation of 1,3 bisphosphoglycerate? Please explain answer.
why is the formation of phosphoenolpyruvate (PEP) a 2 step process in gluconeogenesis (pyruvate --> oxaloacetate --> PEP) but a one step process in glycolysys (PEP-->pyruvate)
7.4
Calculate the GA of hydrolysis of phosphoenolpyruvate (PEP) in
inorganic phosphorus and pyruvate using the following information:
PEP + ADP ATP + H2O piruvato + ATP ADP + P. Keq = 3.2 x 103 AG = -7 700 cal/mol
Phosphoenolpyruvate (PEP) has an extremely high energy phosphoryl bond, meaning that its dephosphorylation produces a substantially greater amount of energy than does ATP. Why is ATP the “energy currency” of the cell but not PEP?
Enolase converts 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP). This reaction has a ΔG’o= + 7.5 kJ/mol, but the reaction occurs readily under normal cellular conditions. What is the ratio of [PEP]/[2PG] at equilibrium, at 25oC? In this case, If 2PG is present within the cell cytosol at a concentration of 6 x 10-3M and the concentration of PEP = 0.10 x 10-3M, what is the ΔG of the reaction at 37oC?
All of the following are precursor metabolites produced during EMP except phosphoenolpyruvate (PEP). glyceraldehyde-3-phosphate. oxaloacetate. pyruvate. Fructose-6-phosphate.
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate (Pi) (a) Is the reaction favorable under standard biochemical conditions? Explain your answer. (b) What is the equilibrium constant (K) of this reaction at 25°C? In cells the direct hydrolysis of PEP rarely occurs. Rather, the hydrolysis of PEP and the reverse hydrolysis of ATP are coupled (c) w rite out the coupled reaction and determine its ΔG at 25°C The hydrolysis of ATP...
Pyruvate kinase catalyzes the last reaction in the glycolytic
pathway:
phosphoenolpyruvate (PEP) + ADP --> pyruvate + ATP
There are four different mammalian forms of pyruvate kinase. All
catalyze the same reaction, but they differ in their response to
the glycolytic metabolite fructose-1,6-bisphosphate (F16BP).
The activity of two forms of pyruvate kinase was measured at
various concentrations of PEP, as shown below. Activity was
measured in the presence (blue circles) and absence (red circles)
of F16BP.
Pyruvate Kinase Form 1...
Pyruvate kinase catalyzes the last reaction in the glycolytic
pathway:
phosphoenolpyruvate (PEP) + ADP --> pyruvate + ATP
There are four different mammalian forms of pyruvate kinase. All
catalyze the same reaction, but they differ in their response to
the glycolytic metabolite fructose-1,6-bisphosphate (F16BP).
The activity of two forms of pyruvate kinase was measured at
various concentrations of PEP, as shown below. Activity was
measured in the presence (blue circles) and absence (red circles)
of F16BP.
Pyruvate Kinase Form 1...
PART A
Correctly order the steps 1-8 as they occur during the transport
of glucose via the phosphotransferase system (PTS):
HPr donates the phosphate group to enzyme IIA (EIIA);
phosphoenolpyruvate is produced as an intermediate of glycolysis;
EI donates the phosphate group to a histidine-rich protein (HPr);
as it enters the cell glucose is phosphorylated by EIIB;
phosphoenolpyruvate donates its high-energy phosphate group to
enzyme I (EI); EIIA passes its phosphate group to Enzyme IIB
(EIIB); glucose is transported across...