streps involve :
proteins involved in homologous recombination in E.coli
Genetics: include pictures please.(: For each of the following steps of recombination at the molecular level,...
Homologous Recombination Lecture Molecular Biology The ability for yeast and E. coli to do homologous recombination easily make it very easy to generate specific mutations. To “knock-out” a gene, you need to transform the cells with a PCR product containing an antibiotic resistance cassette flanked by the genomic DNA sequence where you would like to insert the cassette. Based on our lecture, diagram the process of recombination of the left flank of the following Kanamycin resistance cassette into the E....
1. Homologous recombination can happen between non-identical DNA sequences. T/F? 2. Homologous recombination can happen in_______ a) meiosis b) mitosis c) both 3. Homologous recombination in meiosis has the main purpose of_____ a) DNA repair b) Creating new chromosomes c) Sealing double-stranded breaks 4. Strand invasion usually happens without enzymatic assistance. T/F? 5. When replication fork runs into a nick, it results in a_______ a) single-stranded break b) double-stranded break 6. The invading end is usually a _______ a) 3'...