Homework Answers
By using the formula-
CFU (ml) = number of colonies / dilution factor × volume (ml)
Given - number of colonies = 2500
Volume = 1 ml and TDF = 10-6
CFU (ml) = 2500 / 1 × 10-6 = 25 × 108
So total number of colonies in original sample (before dilution) is 25 × 108.
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I just need help with #4. Thank you!!
4. c. PBLAST I have a bacterial concentration...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial cu...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
After measuring the OD (see question 5) of an S.aureus bacterial culture, you find that the...
After measuring the OD (see question 5) of an S.aureus bacterial culture, you find that the concentration is too high and perform a 1:1 dilution. In order to reach a final volume of 1000uL in your cuvette, what combination of bacterial culture and fresh media must be combined to achieve the desired dilution? If the OD after the dilution is 0.54, what was the undiluted OD? (question 5: After you have grown a culture of E. coli bacteria, you measure...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
In order to count cells, the culture must be duted The technique o serialul precise and...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
I have questions 1-5. I just need help with number 6. Thank you! Assume that the...
I have questions 1-5. I just need help with number 6. Thank
you!
Assume that the concentration of the glucose standard sample is 1.200 mg/ml. Use the following results to perform your calculations: 1. Calculate the concentration of glucose in tubes 1 through 5 (in mu g/ml). 2. Make graphs of A_ vs. concentration and A_ vs. amount (in mu g). It is suggested that you use the program module Excel in Microsoft Office. Instructions are provided in Statistical Calculations...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
I have questions 1-5. I just need help with number 6. Thank
you!
Assume that the concentration of the glucose standard sample is 1.200 mg/ml. Use the following results to perform your calculations: 1. Calculate the concentration of glucose in tubes 1 through 5 (in mu g/ml). 2. Make graphs of A_ vs. concentration and A_ vs. amount (in mu g). It is suggested that you use the program module Excel in Microsoft Office. Instructions are provided in Statistical Calculations...
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