Question

5. If you loaded 0.2 ug of molecular weight markers with the following sized bands, how many ng of DNA would be in the 0.5 kB band? Circle your answer and include units. 14 kB 5 kB 500 bp 4.5 kB 2000 bp 24 kB 2.3 KB 5050 bp How many ng would will be in the top band?

Answer 1

I have loaded 200ng of molecular weight marker. If we consider that the molecular weight markers are present at equal proportion, then

the amount of 500bp ladder present is (200/8)= 25ng of 500bp DNA ladder

In the top band also there will be 25ng of DNA.

(P.S. Distribution in the proportion of different size DNA marker in the mix is not provided in the question. So I considered that they are present in equal prportion.)