Compare the information obtained by Northern Blots and Microarrays: when is one technique preferred over the other?
Answer- The northern blot technique is used to detect a sequence of mRNA encoded by a specific gene. cDNA is used in northern bloting.
In microarray technique the intensity of specific sequence of nucleotides is measured. The cDNA is hybridized by using labelled DNA chips.
The northern blot technique is used to detect the sequence where we have the mRNA sequence while the microarray technique is used to detect specific cDNA sequence.
Compare the information obtained by Northern Blots and Microarrays: when is one technique preferred over the...
Compare the information obtained by Northern Blots and Microarrays: when is one technique preferred over the other?
Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast cells, comparing their growth in two different sugars, glucose and galactose. One student is comparing expression of the gene HMG2 under these two conditions. Which technique(s) could he use and why? Another student wants to compare expression of all the genes on chromosome 4, of which there are approximately 800. What technique(s) could she use and why?
Compare and contrast centralized and D centralized registration why might want to be preferred over the other? Describe an instance
b) What is the main purpose and requirement of REFS O and REFSİ bit in ADMUX register for using ADC in AVR? why interrupt technique is preferred over poling technique in AVR programming? Why lower byte TCNTIL of timer 1(16-bit timer counter register) is required to be read first then TCNT1H? b) What is the main purpose and requirement of REFS O and REFSİ bit in ADMUX register for using ADC in AVR? why interrupt technique is preferred over poling...
What is epidemiology? Compare the type of information obtained from epidemiological studies to that obtained from human intervention studies.
Compare and contrast the techniques (Evaporation, Filtration, Crystallization). When is each technique appropriate? What are the advantages vs disadvantages? Why was the vanillin exposed to two different techniques? Could we have isolated the vanillin using a different technique? Why or why not?
ча 5. When would a wound culture be obtained to determine the appropriate antibacterial agent? Explain your answer. 6. What are the similarities of amikacin to other aminoglycosides? 7. Wound one type of aminoglycoside be preferred over another? Explain your answer.
4. In future lectures we will describe a technique known as Northern blotting that can be used to detect RNA transcribed from a particular gene. Briefly, in this method a specific RNA is detected using a short segment of cloned DNA as a probe. The DNA probe, which is radioactive, is complementary to the RNA that the researcher wishes to detect. After the radioactive probe DNA basepairs to the RNA, the RNA is visualized as a dark (radioactive) band on...
Please write an essay for the following information for the Subcellular fractionation technique: Purpose - Brief description (one to two sentences) of the purpose of this method (ie. what is the general purpose for this technique? When would it be used?) Background – This section will include a description of the concepts and theories that are needed to understand this technique. Method description – this section will include an overview of the steps involved in carrying out the technique. This...
i. Provide a definition of archival research. (4 marks) ii. Compare and contrast how information obtained from archival research might be analysed in a qualitative study and in a quantitative study that uses companies’ annual reports. (you do not need to refer to an actual study) (8 marks) PLEASE ANSWER ACOORDING TO THE MARKS. AND PLEASE DONT COPY OTHER ANSWER