Ans: This is the simplest way engineered the promoters and genes. Promoter and gene of Parvalbumin joined along with promoters and genes of tTA and mCherry respectively. tTA produced to promote the promoter of gene mCherry.
The possible side effect is the production of tTA.
II Best Wishes II
Look at the expression/reporter constructs depicted below. Each gene is named (and individually colored), and each...
Visualizing in live cells (6 pts + 1 bonus) Look at the expression/reporter constructs depicted below. Each gene is named (and individually colored), and each one has its own promoter (also individually colored). Note that the cassette labeled "D" is illustrated under two different conditions (Di and Dii). Glutaminase Parvalbumin tTA mcherry mcherry mCherry 1. Is mCherry normally expressed? le, in it's natural state, is it "on" or "off"? (0.5) 2. Why is the green hexagon molecule important in Dii?...