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Visualizing in live cells (6 pts + 1 bonus) Look at the expression/reporter constructs depicted below....
Look at the expression/reporter constructs depicted below. Each gene is named (and individually colored), and each...
Look at the expression/reporter constructs depicted below. Each gene is named (and individually colored), and each one has its own promoter (also individually colored). Note that the cassette labeled “D” is illustrated under two different conditions (Di and Dii). Α. Glutaminase Parvalbumin tTA mcherry mcherry 4. Keep in mind that the components of each construct (promoter and gene) can be engineered using molecular tools, such that promoters and genes can be cut and pasted, and rearranged. If you wanted to...
1. Gene B is usually expressed only in skin cells. To learn about the mechanism by...
1. Gene B is usually expressed only in skin cells. To learn about the mechanism by which expression of the gene B is regulated, you make clones that contain a GFP reporter and various parts of the upstream and downstream intergenic regions of genomic DNA that normally surround gene B (black lines) as shown in the figure below. The resulting clones were introduced into frog skin cells growing in the lab and levels of GFP expression was monitored by measuring...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
molecular biology Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory r...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
Assignment 1. - Word File Insert Design Layout References Mailings Review View Tell me what you...
Assignment 1. - Word File Insert Design Layout References Mailings Review View Tell me what you want to do Sign in Share Home Cut Find- Aria E Copy Paste Select Editing Format Painter Clipboard Font Paragraph Styles PANCREAS LENt NEURAL TUBE RETINA CORNEA 3 4 イ乙 5. (2) The diagram above shows the a gene with its regulatory sequences. Exon sequences are orange, and enhancer sequences are green, the promoter is red. The enhancers direct gene expression differentially in the...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
1. According to the paper, what does lactate dehydrogenase (LDH) do and what does it allow...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Read the attached article and answer the following questions. 1. What is the role of potassium...
Read the attached article and answer the following
questions.
1. What is the role of potassium in blood pressure?
2. Looking back at last weeks Super Tracker report - do you meet
the 4700mg daily target for potassium?
3. Do you meet the 400mg daily target for magnesium?
4. Research shows that hypertension and type 2 diabetes can be
prevented by dietary choices. List 3 ways that employers,
government, doctors, etc. (you can use another group ) can either
motivate...
Read about Cokes strategy in Africa in the article below and discuss the ethics of selling...
Read about Cokes strategy in Africa in the article below and discuss the ethics of selling soft drinks to very poor people. Is this an issue that a company like Coke should consider? Africa: Coke's Last Frontier Sales are flat in developed countries. For Coke to keep growing, Africa is it By Duane Stanford Piles of trash are burning outside the Mamakamau Shop in Uthiru, a suburb of Nairobi, Kenya. Sewage trickles by in an open trench. Across the street,...
Look at the expression/reporter constructs depicted below. Each gene is named (and individually colored), and each one has its own promoter (also individually colored). Note that the cassette labeled “D” is illustrated under two different conditions (Di and Dii). Α. Glutaminase Parvalbumin tTA mcherry mcherry 4. Keep in mind that the components of each construct (promoter and gene) can be engineered using molecular tools, such that promoters and genes can be cut and pasted, and rearranged. If you wanted to...
1. Gene B is usually expressed only in skin cells. To learn about the mechanism by which expression of the gene B is regulated, you make clones that contain a GFP reporter and various parts of the upstream and downstream intergenic regions of genomic DNA that normally surround gene B (black lines) as shown in the figure below. The resulting clones were introduced into frog skin cells growing in the lab and levels of GFP expression was monitored by measuring...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
Assignment 1. - Word File Insert Design Layout References Mailings Review View Tell me what you want to do Sign in Share Home Cut Find- Aria E Copy Paste Select Editing Format Painter Clipboard Font Paragraph Styles PANCREAS LENt NEURAL TUBE RETINA CORNEA 3 4 イ乙 5. (2) The diagram above shows the a gene with its regulatory sequences. Exon sequences are orange, and enhancer sequences are green, the promoter is red. The enhancers direct gene expression differentially in the...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
Hagrid has purified a protein from unicom sweat and called UNI-1. He has then doned the Unit gone and inserted in rat zygotes (by microinjection) a recombinant construct containing the Uni-1 coding sequence its entire 5 UTR region, covering both the minimal promoter and a series of cutative regulatory als elements. The mutantrats develop glorious, luminous hair. Hagrid has also discovered that the UNI-1 protein binds very tightly to DNA and hypothesizes it is a transcription factor that regulates proliferation...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
Read the attached article and answer the following
questions.
1. What is the role of potassium in blood pressure?
2. Looking back at last weeks Super Tracker report - do you meet
the 4700mg daily target for potassium?
3. Do you meet the 400mg daily target for magnesium?
4. Research shows that hypertension and type 2 diabetes can be
prevented by dietary choices. List 3 ways that employers,
government, doctors, etc. (you can use another group ) can either
motivate...
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