For a nonpolar solute, why does the mobile phase strength increase as the solvent becomes less polar in reversed-phase chromatography?
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For a nonpolar solute, why does the mobile phase strength increase as the solvent becomes less...
Select the correct characteristics describing normal-phase and reversed-phase chromatography. Normal-phase chromatography Reversed-phase chromatography (a) Polarity of phases: Stationary phase is polar Stationary phase is non polar b) Eluent strength of solvent. Increases as solvent becomes more polar. Increases as solvent becomes less polar c) Nature of solutes. Polar Non-polar d) Nature of solute interaction. More soluble in mobile phase as the polarity of the mobile phase increases. More soluble in mobile phase as the polarity of the mobile phase decreases....
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00, circle which of the following compounds would elute first and briefly describe why: Propanoic acid (CH3CH2COOH, PK. = 4.87) or Propylamine (CH2CH2CH2NH2, pks = 3.43). b. If one uses gradient elution with normal phase chromatography, would it be better to go from a less polar to a more polar solvent or a more polar to a less polar solvent? Why?
3. There are two major components in the chromatography experiment: the solvent system (mobile phase) and the paper (stationary phase). Which of these major components is more polar phase and which is less polar phase? Explain using Lewis structures. (please PLEASE use the lewis structures, thats the part of the question I don't have yet!) 4. What parts of the molecules in the stationary phase will interact with the molecules that are being separated? Please answer this question using the...
A solvent passes through a chromatography column in 2.85 min, but the solute requires 9.43 min. What is the retention factor, k?k= What fraction of the time does the solute spend in the mobile phase in the column? tfraction in mobile phase= The volume of the stationary phase is 0.115 times the volume of the mobile phase in the column (Vs=0.115Vm). What is the partition coefficient, K, for this system? K=
A solvent passes through a chromatography column in 2.18 min, but the solute requires 8.45 min. What is the retention factor, k? k= What fraction of the time does the solute spend in the mobile phase in the column? I fraction in mobile phase = The volume of the stationary phase is 0.102 times the volume of the mobile phase in the column (Vs = 0.102Vm). What is the partition coefficient, K, for this system? K=
Why would it be unwise to use 100% ethyl acetate as the first mobile phase you test when determining the best mobile phase to separate the mixture of analgesics in Procedure? Why do polar compounds move slower up the silica TLC plates than nonpolar compounds? Why does increasing the polarity of the mobile phase increase the rate at which all compounds move up the plate?
A solvent passes through a chromatography column in 3.41 min, but the solute requires 9.13 min. What is the retention factor, k? k= What fraction of the time does the solute spend in the mobile phase in the column? fraction in mobile phase The volume of the stationary phase is 0.101 times the volume of the mobile phase in the column (V = 0.101V.). What is the partition coefficient, K, for this system?
Why is water called the universal solvent? A. Water can act as a solute in many different solutions. B. Water is a nonpolar substance that dissolves polar substances. C. Water is a polar substance that dissolves nonpolar substances. D. Water can act as a solvent in many different solutions.
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile phase. Match each of these terms to what is was in out experiment. Stationary Phase ____ a) The solvent Mobile Phase ____ b) Silica Analyte ____ c) One of the analgesiscs Problem 2 Complete the sentences Analytes on a TLC should have a(n) _________ Rf in a less polar solvent. Analytes on a TLC should have a(n) _________ Rf in a more polar solvent....
In liquid chromatography, the solvent (mobile phase) composition often changes with time during each run. What parameter usually changes with run time in gas chromatography and why?