Question

.1)a) Why was it important that the –pGLO bacteria were found growing on the LB plate? Why

would their absence create problems in interpreting our results?

b) What two conclusions (one per plate) should you draw from the observation that –pGLO

bacteria did not grow on the LB/amp plate, but that some +pGLO bacteria did?

c). Why did we observe bacteria colonies that glowed under UV light when we inoculated

+pGLO bacteria on an LB/amp/ara plate, but not when we inoculated +pGLO bacteria on

an LB/amp plate?

4. If you inoculated –pGLO bacteria on an LB/ara plate, a) would you expect them to grow and

b) would you expect the colonies to glow if they did grow? Explain your answer.

5. If you inoculated +pGLO bacteria on an LB/ara plate, a) would you expect them to grow and

b) would you expect the colonies to glow if they did grow? Explain your answer.

Answer 1

Q. 1a. This plate contain nutrient medium for bacteria, ampicillin and antibiotics are added. They will glow , If they expose the colonies, to uv light.

b . In this lab :- there are various combinations of ampiallin, arabinose, without the lab / pGlo , there is antibiotics resistance to bacteria, from that bacteria will not glow in dark.The growth of bacteria will stop.

If this plate have sugar, like arabinose:- then bacteria will both glow and grow.

+pGLO LB/ amp and - pGLO LB/amp will whitish.

And +pGLO LB/ amp/ ara whitish in normal condition and green in u.v light.

C. If there is positive colonies, by presence, of pGLo Lab/amp, or pGLa lab/amp/ ara plate, and absence of negative plate, then this colonies will glow green.

If there is absence of pGLA lab/amp , pGLa lab/amp/ ara or adding plasmid or not adding bacteria to pGlo. They will not glow.

Q.4. a. In - pGlo lb/ara plate , in this the started colonies will not grow here,

b. If there is + pGLO plate the colonies will transformant.

Q.5. a. If bacteria grow on lb/amp plate they are resistant to ampicillin. If there no bacteria growth take place then , they were sensitive to ampicillin.