Question

gene transfer like conjugatione 5. What is calcium chloride transformation? What does it accomplish? Calcium Chloride trensformation increases the ability of a bacteria call to in corporale plasmid DNA which callows it to be genetically frensformed. If accomplishes binding op plasmid DNA IV. 6. Why would you expect to find cell growth on the Luria nutrient agar plate? (LB plate)? 7. Did you expect to find cell growth on the ampicillin Luria plate? Explain WHY or WHY NOT. (LB/amp plate with and without pGlo) 9. How does Heat Shock affect plasmid transformation?

Answer 1

Answer6) There is a lawn formation on LB Agar plate,if there is no antibiotics present all the cells grow even without plasmids

Answer7)Control plates that is LB plates without ampicillin- In this plates one can observe bacterial colonies without any restriction whereas, treated cells that is LB plates with ampicillin observes specific bacterial lawns that is only specific variety of bacteria will grow that has been innoculated.

Answer 9)Heat shock is a treatment (physical) that assists in bacterial cell lysis, the treatment may be Cacl2 treatment/sonication/high temperature radiation.

when heat shock is provided to bacteria following process takes place (a) when foreign DNA is inserted into bacteria via heat shock this is known as Transformation (b)Bacteria is grown on antibiotic resistant plates so that only single colonies can grow (c) The bacterial colonies possessing appropriaate plaasmids can be propogated at large scale to make proteins