Question

A researcher wants to selectively stimulate (depolarize) serotonergic neurons that project to the prefrontal cortex. Using a mouse model, what technique should be used? O a. deep brain stimulation in the prefrontal cortex express an inhibitory receptor in serotonin neurons and then administer CNO directly into the prefrontal cortex O c. express Channelrhodopsin-2 in serotonin neurons and then administer CNO directly into the prefrontal cortex express Channelrhodopsin-2 in serotonin neurons and then deliver blue light (fiber optics) into the prefrontal cortex Oe. express Halorhodopsin in serotonin neurons and then administer green-yellow light (fiber optics) into the prefrontal cortex QUESTION 47 A postsynaptic receptor is activated by a neurotransmitter allowing an influx of Cl- into the postsynaptic neuron. What happens to the postsynaptic membrane potential? O a. ionotropic b.metabotropic O c. inhibitory postsynaptic potential (hyperpolarization) d. excitatory postsynaptic potential (depolarization) O e. not enough information to determine

Answer 1

Ans 46. Option d

Very brief (millisecond) pulses of blue light can be used to induce single action potentials in Channel rhodopsin-2-expressing neurons, and spiking activity driven by the activation of this opsin can be controlled with high precision at frequencies approaching 30 spikes per second.

Ans 47. Option C

An inhibitory postsynaptic potentials (IPSP) is a temporary hyperpolarization of postsynaptic membrane caused by the flow of negatively charged ions (i.e. Cl-) into the postsynaptic cell.