True or false. “A negative gram stain in a patient experiencing weight loss, chronic cough, and...
Homework Answers
Gram staining is a staining technique developed by Hans Christian Gram. It differentiates between gram positive (which stain purple) and gram negative (which stains pink) bacteria.
|
Gram positive bacteria |
Gram negative bacteria |
|
Contains a thick peptidoglycan layer. |
Contains a thin peptidoglycan layer. |
|
Multi-layered peptidoglycan layer present. |
Single layered peptidoglycan layer present. |
|
Retains the stain after washing with alcohol. |
Doesn’t retains the stain after washing with alcohol. |
|
Outer lipopolysaccharide layer absent. |
Outer lipopolysaccharide layer present. |
|
Teichoic acids present. |
Teichoic acids absent. |
|
More susceptible to antibiotics. |
More resistant to antibiotics. |
In this procedure,
- A smear of bacterial culture is made on the slide and heat fixed.
- Stained with crystal violet.
- Washed with water and then with 95% ethanol.
- Stained with safranin.
- Washed with water.
- Observe under the microscope.
Gram staining just tells whether the smaple contains a gram negative or positive bacterium and has not to do anything with whether or not the patient is diseased. Just based on a gram negative stain of a sample, one cannot conclude whether the patient is suffering from a disease.
It maybe used to confirm, in case of a patient is suffering from a bacterial infection, to know whether ornot the bacteria is gram negative or gram positive.
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True or false. “A negative gram stain in a patient experiencing
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CHIEF
COMPLAINT: Cough and fever for four days
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a 68 year old man who developed a harsh, productive cough four days
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Case Study
#1
CHIEF
COMPLAINT: Cough and fever for four days
HISTORY: Mr. Alcot is
a 68 year old man who developed a harsh, productive cough four days
prior to being seen by a physician. The sputum is thick and
yellow. He developed a fever, shaking, chills and
malaise along with the cough. One day ago he developed pain in his
right chest that intensifies with inspiration. He lost 15 lbs. over
the past few months but claims he did not...
A 34 y/o male with a history of tobacco and alcohol abuse (12 cans of beer per day) was admitted to the hospital. Two months prior to admissi have a necrotic in his right upper lobe. He was PPD (purified protein derivative) negative and three sputum cultures were negative for acid fast Mycobacterium spp. He had no risk factors for hum on he was seen at an outside hospital, where he was found to Four weeks prior to admission, he...
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