Question

Trypanosoma brucei is the agent that causes African sleeping sickness in humans bitten by the tsetse fly. Glycolysis is unusual in this organism, providing an opportunity to create drugs that poison the parasite without harming humans. According to Verlinde et al., 2001:
“The seven glycolytic enzymes converting glucose to 3-phosphoglycerate are localized in specialized peroxisome-like organelles called glycosomes… One molecule of each glycerol and pyruvate are produced per molecule of glucose… The net ATP production is … one [molecule].”
Unlike trypanosomes, in most other organisms the following are true: (Select all that apply.)

Glycolytic enzymes are cytosolic.  

Glycolytic enzymes are in the mitochondrial matrix.  

Glycolysis results in two molecules of glycerol.  

Glycolysis results in two molecules of pyruvate.  

Net ATP production is two molecules.  

Net ATP production is four molecules.  

Answer 1

In most of the organisms, glycolysis takes place in the cytoplasm of the cell so the enzymes of glycolysis are present in the cytoplasm of the cell. (glycolysis enzymes are cytosolic)

In glycolysis, one molecule of glucose is breakdown to produce two three-carbon compound pyruvate molecules (Glycolysis results in two molecules of pyruvate)

In glycolysis, four ATPs are produced by the substrate-level phosphorylation, two molecules of ATP are utilized during the conversion of glucose into glucose 6 phosphate, and fructose 6 phosphate into fructose 1,6 bisphosphate, so Net ATP production is two molecules.