Pan QUESTION 2 What does the acronym SDS-PAGE stand for? How is this technique applied within...

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Pan QUESTION 2 What does the acronym SDS-PAGE stand for? How is this technique applied within the lab? Applied as a bioanalyt

Answer 1

Answer #1

SDS PAGE: Sodium Dodecyl sulfate polyacrylamide gel electrophoresis.

SDS PAGE is used for protein separation.

The gel is made with addition of anionic detergent called Sodium Dodecyl sulfate (SDS)

Net negative charges are imparted to the protein samples.

The SDS binding is proportional to the relative molecular mass of the protein sample.

SDS denatures the protein samples into monomeric units

The proteins with lower molecular weight travels fasted in SDS page and are found closer to the anode compared to samples of higher molecular weight.

Used for:

Distinguishing and isolating protein from a sample mixture.

Purification and protein assessment.

Assessing molecular weight of proteins

As preliminary stage for Western-Blotting.

Answer 2

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