Question

(a) empty vector (b) my IIA (C) my IIB View larger image

help with these questions please !

Answer 1

Ans. a (i): Cells with empty vector is the control for this experiment. The effect of vectors carrying the gene of interest (my IIA & my IIB) will be compared against the cells transfected with empty vector.

Ans. a (ii): Below are the numbers of cells adhered to the culture dish

• Number of cells with empty vector – 12
• Number of cells with my IIA vector – 80
• Number of cells with my IIB vector – 12

Conditions	No. of cells adhered	% w.r.t empty vector
Number of cells with empty vector	12	100
Number of cells with my IIA vector	80	667
Number of cells with my IIB vector	12	100

Ans. a (iii) Below graph is the representation of experimental results

667 % increase in cell number w.r.t. empty vector 100 100 Number of cells with empty vector Number of cells with my 11B vector Number of cells with my IIA vector experimental conditions

Ans. a (iv) Over expression of myosine isoform - my IIA has significantly increased the cell adhesion whereas the isoform – IIB does not have any impact on the adhesion capability. These results clearly suggest the importance of my IIA in cell adhesion.