Question

sidering how cellular metabolism (catabolism and anabolism) works, explain why a bacteria. faster on this complex medium (glucose, beef extract, yeast extract, peptone, and water) vs. this fined/synthetic medium (sucrose, KzHPO., KH PO, (NH), HPO4, MgSO4, FeSO4, MnSO4, and water 6. Explain why Conside 4. Complement proteins, which are part of your immune system, can for large pores in the cytoplasmic 5. Escherichia coli is a facultative acrobe. Imagine a culture of E coli growing at 37°C in a complex broth medium with abundant glucose. In which of the following incubation conditions would the cells grow at a faster rate: (a) loosely capped flask on a shaking incubator (to keep the broth stirring) or (b) tightly sealed flask without shaking? Explain. bo try sealed flask wl out sharing to

Answer 1

Q 3: cellular metabolism:-1. cellular metabolism in bacteria focuses on reaction by which reactant(substrate) molecule are broken down into product and generate energy. Based on the process of oxidation and dissimilation. It is basically study of uptake and utilisation of the inorganic or organic compounds required for growth ( Assimilation).

2. Bacteria culture grows faster in glucose using leucine and acetate in ergosterol . After adding glucose it results in an initial lag phase after that exponential phase , in that bacterial growth decrease. The presence of glucose express the inducible enzyme synthesis(glucose effect) ,( transient),( permanent catabolite repression).

3. Bacteria evolved in environment where sugar and salt concentration is same or lower inside the cell.

Q.4:- There are pore forming toxin which are cytotoxin protein . These generally distrupts host cell membrane. Funtions involved distruption of epithelial barrier function, eruption of bacteria growth.

Q.5:- high oxygen transfer rate are required for many shake flask . They depend on 1: physical condition ( speed of shaking , flask type ,clousure tyoe) . 2. Rheological property of culture.

The baffle flask increase oxygen.

The use of cotton to close affect oxygen supply they interfere the oxygen supply.