Question

A) The genome of Z. mobilis was not sequenced when this work was originally conducted. Use IMG or BLAST (or MetaCyc) to find whether the following enzymes are encoded in the Z. mobilis ZM4 genome and provide the locus tag / gene name (beginning with “ZMO”) of each, if present: transaldolase, transketolase, phosphoglucose isomerase (glucose-6-phosphate isomerase), phosphofructokinase, fructose-bisphosphate aldolase, and triosephosphate isomerase. Explain why Z. mobilis cannot use the EMP pathway to degrade glucose.

B) Z. mobilis uses the Entner-Doudoroff pathway for ethanol fermentation. As a result, the expected ATP yield is only 1 ATP per molecule of arabinose. Explain why conversion of 6 molecules of arabinose to ethanol and carbon dioxide would only yield 6 molecules of ATP. Although this is less beneficial for the bacterium, it is better for ethanol production. Why?

Answer 1

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& deare dehyhre Glyceral dehyd-zup po kelos debny gluconale aldelare Pyruvale 2 NADH • NA 2 telhanel. Ethand Fermentation in zgomonas mobilis. i z Mobilis makes use of it miquely for anaerobic metabolinm. The low ATP greld Penulan in a low cell mads and the politiet fe higher Ethanol grelds. (Page 2)
Z mobilis all genes for the enzymes of the Glycolytic pathway are present except for phosphofructokinase and without this glycolysis is blocked.

Even more confirmidingly z mobilis is missing most of the enzymes for the Penrose phosphate pathway.

Z mobilis lacks genes for 2-oxoglutarate dehydrogenase and Malate dehydrogenase and Consequently has an incomplete TCA cycle.

This doesn't block amino acid synthesis for the most part. Because other pathway apparently function in this aspect.

Deficiency in Glycolysis and Penrose phosphate pathway greatly constrain the abiyof zmobilis to assimiother sugars.

Infact it was precisely object of adding the capacity for Close and arabinose metabolism.