Question 4: (1 point) Name one enzyme you would introduce into Z. mobilis to decrease the...
A) The genome of Z. mobilis was not sequenced when this work was originally conducted. Use IMG or BLAST (or MetaCyc) to find whether the following enzymes are encoded in the Z. mobilis ZM4 genome and provide the locus tag / gene name (beginning with “ZMO”) of each, if present: transaldolase, transketolase, phosphoglucose isomerase (glucose-6-phosphate isomerase), phosphofructokinase, fructose-bisphosphate aldolase, and triosephosphate isomerase. Explain why Z. mobilis cannot use the EMP pathway to degrade glucose. B) Z. mobilis uses the Entner-Doudoroff...
Metabolic Pathway Engineering Problem Set 5 Engineering a Fermentation System: Fermentation of plant matter to produce ethanol for fuel is one potential method for reducing the use of fossil fuels and thus the CO2 emissions that lead to global warming. Many microorganisms can break down cellulose then ferment the glucose to ethanol. However, many potential cellulose sources, including agricultural residues and switchgrass, also contain substantial amounts of arabinose, which is not as easily fermented. Escherichia coli is capable of fermenting...