Question

38. Alice has just begun working in a protein biochemistry lab particular protein that was isolated from human cells. He om a protein biochemistry lab where her job is to study the function of a ands her a tube containing the pure protein to work with and cautions her to keen it at pH 7.0 in a buffered solution at In the process of working with the protein, Alice made a miscal the solution the protein was in to a pH of 8.0! the protein, Alice made a miscalculation in her buffer recipe, which altered 7.0. (2) precisely how much higher or lower the proton concentration is at pH 8.0 than it was a

Answer 1

38. Proton concentration will be lower in pH8 than in pH 7

pH 7 will have proton concentration of 1* 10-7 M

PH 8 will have proton concentration of 1*10-8 M

Concentration difference = 1*10-1 M or 0.1 M

39. Changing the pH to 8 results in proton loss means in the primary structure it looses hydrogen ions and than in the secondary tertiary structures protein may be denatured due to hydrogen, ionic bond disruption occurs due to pH change

Protein may not be functional if it loses native structure that holds the active site of the enzyme and if the protons participate in enzyme catalysed reaction due to pH change