In figure, the marker gene(s) used for selecting the plasmid is:
B) ampR
It is useful to see whether the bacteria are successfully transformed or remain non-transformed. If not transformed, when grown on medium containing ampicillin bacteria die due to lack of ampicillin resistance.
In Figure 9.5 (see attachment), the marker gene(s) used for selecting the Plasmid is lacz Hindill...
Q5. Figure 2 shows the plasmid PGL4.83[hRlucP/Puro]. Based on Figure 2, answer the following: Synthetic poly(A) Amp' Sall 2599 ori Sfil Acc651 Kpnl EcolCRI Sacl Nhel Xhol Bglli D Synthetic poly(A) PGL4.83[hRlucP/Puro) Vector (4815bp) Sfil Hindlll hRlucP Puro SV40 early enhancer/ promoter EcoRI 1033 SV40 late poly(A) signal 5141MA 1431 BamHI Figure 2 (Source: httpwww.biofeng.comzaitīburup GL4.83.html 1) There are two synthetic polv(A) sites flanking the ori and Amp genes. State the technique used to achieve these poly(A) sites. Briefly elaborate...
The extracted plasmid DNA is pUC19. Look up the plasmid map and include in your pre-lab as well as your report. a. What is the location of the BamH1 restriction site? Xmn1 restriction site? (sequence location by number). How many of these sites exist each? b. If single digestions were performed (one restriction enzyme) with BamH1 and Xmn1, respectively, how many fragments would form, and what would be the sizes of each of these fragments? c. If a double digestion...