Question

5. The measured Hill coefficient for a binding to hemoglobin under Physiologie conditions, which has four binding site of for a, is only about 3. Why is this?

please answer in details this biophysical chemistry question

Answer 1

The measurement of the ligand affinity for a specific receptor is referred to as the Hill coefficient.

It expresses the fraction of the saturated macromolecule in the existence of a specific ligand for showing its role.

The measurement of the hill coefficient is 3 for hemoglobin. It exhibits positive cooperativity.

Even though the four binding regions of the tetrameric hemoglobin is saturated by the oxygen molecule. By the use of Hill equation for a macromolecule, only the ligand molecule percentage is required for its role but not the exact number of ligand molecules.

P+nL PL. - Equation (1) P= Partial pressure of O, For this equation (1) Dissociation constant [P][L]" K TPL) = Fraction of engaged binding sites can be determined by using the hill equation and not the exact number of oxygen. [Ln K + [L] 1