Question

27. Tou nave isolated the gene for a protein called nuculin (about O KDA) normally localized in the nucleus. Based on the protein sequence analysis using a computer program, you find a potential nuclear localization signal (NLS). You want to know whether this potential NLS is sufficient to bring nuculin into the nucleus. Please design experiments to address your question. You can use what you learned in Biol 203 or Biol 300.

Answer 1

If you designed to synthesise GFP tagged nuculin protein by genetic engineering , then this protein will go to the nucleus only when it contain NLS.

But if you mutate the the NLS then ,signal will not come from nucleus rather it will come from other positions.

Thank you