Question

Suppose you want to amplify the following bracketed sequence. — 3'-AATCG [TATCAGCAGCAGCAG]TGACT-5 – to - 5'-ITAGC[ATAGICGICGICGTC]ACIGA-3

Which of the following primer sets would be capable of generating an amplicon consisting of nucleotides only found in the bracketed region?

Note: More than may answer can be selected

	a.	5’-GAC-3’ and 5’-ATA3’
	b.	5’-TAT-3’ and 5’-CTG-3’
	c.	5’-TAT-3’ and 5’-GTC-3’
	d.	5’-TCA-3’ and 5’-TTA-3’
	e.	5’-ATA-3’ and 5’-TAT-3’

Answer 1

I believe the correct answer to be:

Option A) 5’-GAC-3’ and 5’-ATA3’

This is the only combination that can replicate the desired fragment of DNA.

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