A. Give the nucleotide sequence of the newly synthesized DNA strands
B. Identify the leading and lagging strand
Homework Answers
the replication fork is shown in the image.
the template strand having 3` to 5` polarity in the direction of movement of the replication fork is the template strand for the synthesis of the leading strand, and the other template strand having polarity 5` to 3` in the direction of movement of the replication fork is the template strand for lagging strand.
DNA polymerase adds new nucleotides complementary to nucleotides in the template strand,
A is complementary to T and G is complementary to C.
a) sequence of newly synthesized strands is 5`-ATCTGCTAATTTA-3` and
5`-TAAAATTGCAGAT-3`
b) so the leading strand is 5`-ATCTGCTAATTTA-3`
the sequence of the lagging strand is
5`-TAAAATTGCAGAT-3`
Add Answer to:
A. Give the nucleotide sequence of the newly synthesized DNA
strands
B. Identify the leading and...
The lagging strand in DNA replication?: (A) is synthesized after the leading strand. (B) causes the...
The lagging strand in DNA replication?: (A) is synthesized after the leading strand. (B) causes the formation of Okazaki fragments in the leading strand. (C) is a consequence of replicating both strands of template DNA at a single replication fork. (D) requires its own replisome.
RNA primers on the leading and lagging strands
The diagram below shows a replication bubble with synthesis of the leading and lagging strands on both sides of the bubble. The parental DNA is shown in dark blue,the newly synthesized DNA is light blue, and the RNA primers associated with each strand are red. The origin of replication is indicated by the black dots on theparental strands.Rank the primers in the order they were produced. If two primers were produced at the same time, overlap them
Draw a figure of one double stranded DNA molecule that has initiated replication and produced two...
Draw a figure of one double stranded DNA molecule that has initiated replication and produced two okazaki fragment in each lagging strand. The figure must include both template strands, all newly synthesized DNA molecules on both leading and lagging strands, all RNA primers, direction of chain growth for each fragment/strand indicated with arrowheads, direction of replication forks. Each molecule must be labeled with the origin of replication on both strands, leading and lagging strands labeled, template or newly synthesized, DNA...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
A single replisome complex can simultaneously synthesize both the leading and lagging strands of DNA within...
A single replisome complex can simultaneously synthesize both the leading and lagging strands of DNA within a moving replication fork. (a) Why is this challenging? (b) Use the “trombone model” analogy to explain how a single replisome synthesizes the lagging strand at the same time the leading strand is being made.
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded...
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
What figure correctly depicts the direction of replication of the leading and lagging strands at replication...
What figure correctly depicts the direction of replication of the leading and lagging strands at replication fork? (All lines with arrows show the newly synthesized DNA and indicate the direction of polymerization. The 5' and 3 designations are indicated on the template DNA.) A) A B) B C) C D) D E) E
Sketch these DNA models, recording the nucleotide sequence of the strands and labeling the 3’ and...
Sketch these DNA models, recording the nucleotide sequence of
the strands and labeling the 3’ and 5’ ends of each.
1
in the figure below, the dashed line represents a newly synthesized DNA strand while the solidines...
in the figure below, the dashed line represents a newly synthesized DNA strand while the solidines represent parental/template DNA strands. The polarity for one parental strand is given the direction of the replication fork is indicated with an arrow. With respect to this replication fork, in which direction would helicase be working? A B C D Direction of replication
Give the sequence of the complementary strand of the following DNA of one DNA strand of...
Give the sequence of the complementary strand of the following DNA of one DNA strand of a DNA double helix is the (6 pts) nd of the following DNA strands. The nucleotide sequence a. 5'-GGATTTTTGTCCACAATCA-3' b. 3'-TTCGAGTCCAAGTCGTACTA-S or magnesium chloride (MgCl) is dissolved in 2.40 L of water. (Molar mass of MgCl2 -.11g/mole) (15 pts) a. What is the molarity of solution? b. How many moles of MgCl, are contained in 1.76L of solvent? C. How many liters of solvent...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
What figure correctly depicts the direction of replication of the leading and lagging strands at replication fork? (All lines with arrows show the newly synthesized DNA and indicate the direction of polymerization. The 5' and 3 designations are indicated on the template DNA.) A) A B) B C) C D) D E) E
Sketch these DNA models, recording the nucleotide sequence of
the strands and labeling the 3’ and 5’ ends of each.
1
in the figure below, the dashed line represents a newly synthesized DNA strand while the solidines represent parental/template DNA strands. The polarity for one parental strand is given the direction of the replication fork is indicated with an arrow. With respect to this replication fork, in which direction would helicase be working? A B C D Direction of replication
Give the sequence of the complementary strand of the following DNA of one DNA strand of a DNA double helix is the (6 pts) nd of the following DNA strands. The nucleotide sequence a. 5'-GGATTTTTGTCCACAATCA-3' b. 3'-TTCGAGTCCAAGTCGTACTA-S or magnesium chloride (MgCl) is dissolved in 2.40 L of water. (Molar mass of MgCl2 -.11g/mole) (15 pts) a. What is the molarity of solution? b. How many moles of MgCl, are contained in 1.76L of solvent? C. How many liters of solvent...
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