Question

12. The saccharide binding pockets in lysozyme were labeled A through F. Enzymes such as lysozyme are very specific; lysozyme will hydrolyze the polysaccharide at only one linkage: the linkage bound between A and B, or bound between B and C, etc. (see figure below). From the crystal structure, it was found a) NAG-NAG-NAG binds at sites A-C in the crystal structure, and b) that sites A, C and E could not accommodate NAM; NAM's extra lactyl group is too big to bind in A, C or E pockets of lysozyme. Using this information, label the peptidoglycan monosaccharides in the figure below as either NAG or NAM, and draw an arrow to the possible linkages lysozyme might cut. D A polysaccharide (natural substrate) F enzyme B C D E

Answer 1

1 1 1 1 r NAG-NAM-NAG-NAM-NAG-NAM site of cleavage of glycosidic bond. 3 Hy horolysis Weed'on berseen :D and E site (1) NAG callse binding at A-site will associated posty NAG at binding at Breite will associated poorly. asko (Association constant) is less in bolts conditions.

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