Homework Answers
Conjugation is the process by which the genetic material is passed from F+ or HFr cell to F- cell via sex pili.
F+ cell contains F plasmid, it remains as plasmid it cannot recombine with chromosome. so during conjugation with the F- cell it can the donor strain with F+ plasmid can only contribute F plasmid.
In Hfr cell, F plasmid is recombied with the bacterial chromosome, so during conjugtion with the F- cell the Hfr donor can contribute genes in the bacterial chromosome to the recipient cell depending upon the time of conjugation, if the conjugation is allowed for longer time it pass genes from the chromosome also to the F- cell.
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1. Differentiate between an F+ cell and an Hfr cell in terms of where the F...
What statement about conjugation is true? Group of answer choices A. Hfr strains are more useful...
What statement about conjugation is true? Group of answer choices A. Hfr strains are more useful for genetic mapping than F+ strains because Hfr strains can conjugate with any strain, while F+ strains can only conjugate with F- strains. B. Hfr strains are more useful for genetic mapping than F+ strains because they recombine transferred genetic material in the recipient cell at a higher rate. C. Hfr strains are more useful for genetic mapping than F+ strains because Hfr strains...
1) Choose from the following options to complete the passage below: F-, F+, F', Hfr. A(n)...
1) Choose from the following options to complete the passage
below: F-, F+, F', Hfr.
A(n) ___?___ cell, which has the F plasmid incorporated into the
bacterial genome, can only conjugate with ___?____
cells. If the plasmid recircularizes, carrying some of the
bacterial DNA with it, the cell is now considered to be
___?____.
2) If conjugation occurs between the two cells below, what will
be the genotype of the exconjugant cell?
a) F+ lac+
b) F+ lac-
c) F-...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
QUESTION 12 Which of the following statements about genetic exchange in bacteria is NOT true? A....
QUESTION 12 Which of the following statements about genetic exchange in bacteria is NOT true? A. In conjugation there has to be a physical connection between the donor cell and the recipient cell. B. Antibiotic resistance can be transferred from one bacterial cell to another by conjugation. C. Interrupted conjugation results in the production of Hfr strains. D. Plasmids do not have to integrate into the host cell chromosome in order to be replicated. E. The order of gene transfer...
Need all answers with explanations please The CRISPR/Cas system was identified by microbiologists who were analyzing...
Need all answers with explanations please
The CRISPR/Cas system was identified by microbiologists who were analyzing microbial genomes and discovered bacteriophage genes that were integrated into the bacterial cell's chromosome. b.) Imagine that a bacterial cell's chromosome contains the a CRISPR/Cas spacer in of bacteriophage gene A (A+) and that you can detect that this cell contains the F genetic information by PCR. You do not know if the cell is an F+ cell or an Hfr cell. You set...
Group activity 12 (chapter 12) Group #: Names: 1. A phage that is part of a...
Group activity 12 (chapter 12) Group #: Names: 1. A phage that is part of a bacterial chromosome is said to show: B) immunity C) conjugation. D) lysogeny E) virulence. 2. Different Hfr strains that originate from the same F-positive strain provide different transfer maps of bacterial genes because: A) the F plasmid inserts at random into the bacterial chromosome. B) the F plasmid replaces some chromosomal segments when it integrates C) insertion of the plasmid causes rearrangements of the...
Which of the following is NOT true about bacterial conjugation An F factor drives replication and...
Which of the following is NOT true about bacterial conjugation An F factor drives replication and transfer of a conjugative plasmid The genome of the bacterial chromosome in the donor cells is altered through ectopic recombination Transfer is mediated by a tube-like pilus formed between the adjacent cells Mating cells often break apart before transfer of the entire bacterial chromosome. The transferred DNA can be incorporated in the recipient cell bacterial chromosome via homologous recombination.
Need all answers with explanations please The CRISPR/Cas system was identified by microbiologists who were analyzing...
Need all answers with explanations please
The CRISPR/Cas system was identified by microbiologists who were analyzing microbial genomes and discovered bacteriophage genes that were integrated into the bacterial cell's chromosome. d.) Imagine that a bacterial cell's chromosome contains the a CRISPR/Cas spacer in of bacteriophage gene A (A+) and that you can detect that this cell contains the F genetic information by PCR. You do not know if the cell is an F+ cell or an Hfr cell. You set...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and streptomycin resistant (str). Your recipient cell is F-hmm omg poo' and streptomycin susceptible (str). Below are the results from that interrupted mating experiment. frequency of Hfr genetic characters among str-resistant exconjugants a) If you attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 20 minutes on a complete media plate that contained streptomycin, would you expect to see growth? + X...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks)...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
1) Choose from the following options to complete the passage
below: F-, F+, F', Hfr.
A(n) ___?___ cell, which has the F plasmid incorporated into the
bacterial genome, can only conjugate with ___?____
cells. If the plasmid recircularizes, carrying some of the
bacterial DNA with it, the cell is now considered to be
___?____.
2) If conjugation occurs between the two cells below, what will
be the genotype of the exconjugant cell?
a) F+ lac+
b) F+ lac-
c) F-...
Need all answers with explanations please
The CRISPR/Cas system was identified by microbiologists who were analyzing microbial genomes and discovered bacteriophage genes that were integrated into the bacterial cell's chromosome. b.) Imagine that a bacterial cell's chromosome contains the a CRISPR/Cas spacer in of bacteriophage gene A (A+) and that you can detect that this cell contains the F genetic information by PCR. You do not know if the cell is an F+ cell or an Hfr cell. You set...
Group activity 12 (chapter 12) Group #: Names: 1. A phage that is part of a bacterial chromosome is said to show: B) immunity C) conjugation. D) lysogeny E) virulence. 2. Different Hfr strains that originate from the same F-positive strain provide different transfer maps of bacterial genes because: A) the F plasmid inserts at random into the bacterial chromosome. B) the F plasmid replaces some chromosomal segments when it integrates C) insertion of the plasmid causes rearrangements of the...
Need all answers with explanations please
The CRISPR/Cas system was identified by microbiologists who were analyzing microbial genomes and discovered bacteriophage genes that were integrated into the bacterial cell's chromosome. d.) Imagine that a bacterial cell's chromosome contains the a CRISPR/Cas spacer in of bacteriophage gene A (A+) and that you can detect that this cell contains the F genetic information by PCR. You do not know if the cell is an F+ cell or an Hfr cell. You set...
4. You've run an interrupted mating conjugation experiment with Hfr cells that are hmm* omgpoot and streptomycin resistant (str). Your recipient cell is F-hmm omg poo' and streptomycin susceptible (str). Below are the results from that interrupted mating experiment. frequency of Hfr genetic characters among str-resistant exconjugants a) If you attempted to grow your exconjugants (those recipient cells that underwent conjugation) at 20 minutes on a complete media plate that contained streptomycin, would you expect to see growth? + X...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
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