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No molecule x present 44 4 - Site 1 Site 1 Site 2 Site 2 — Low concentrations of molecule x Site 1 Site 2 High concentrations

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Answer #1

When no molecule of X is pressnt, then A can not bind to the either site on DNA. No.

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The A-X binds to the site 1 at low concentration. This suggests that A-X has higher affinity to bind to Site1 as compared to site2.

Site 1 has higher affinty.

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Site 2 requires much larger concentration of A-X in order to bind to it. Because the A-X binds to site at lower concentration. This suggests that site 2 requires larger concentration as compared to site 1.

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