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9. Voltage clamps are normally used to “clamp” or “freeze” membrane potential at a particular value and then measure the resulting ionic currents. Explain how setting a voltage clamp at the presynaptic terminal to a very positive potential can prevent synaptic transmission (hint: synaptic transmission is triggered using voltage-gated Ca++ channels, and you can assume typical Ca concentrations inside and outside the cell from the table.

Please explain how setting a voltage clamp at the presynaptic terminal to a very positive potential can prevent synaptic transmission based on the table below using the Ca++ concentration given

En [] in mM (mv) Inside Outside neuron neuron 150 10 12 120 50 1.25 250 Na+ CI Ca++ 100

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when action porential develops in presynaptic membrane, it becomes permeable for catt. catt enter presynaptic membime and neu

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