Question

lab:extraction of DNA from onion tissue, and strawberries

Do Onions, strawberries and Bananas Have ONA? EDVOKIE *5-75 Study Questions 1. Describe the appearance of the isolated DNA. 2. What is a nucleus? 3. What are nucleotides? 4. What are chromosomes? 5. What does cell lysis mean? 6. Why did the alcohol layer on top of the DNA solution? 7. Why was it important not to mix the alcohol and DNA solutions? 8. What properties of DNA allow spooling? 9. What difficulties did you have in spooling? Why? 10. How many chromosomes do humans have?

Answer 1

Ans.1. Isolated dna appearance is based on the dye used like ethidium bromide is commonly used so orange red appearance found

Ans.2.nucleus is the part of the cell containing genetic material encloses by nuclear membrane perform all vital function.

Ans.3.nucleotide is the unit of dna or rna comprised of N-base & phosphate&sugar.

Ans.4.chromosome is formed by the condensation of the polynucleotide of the dna or you can say that the dna wrapped on the histone protein in such a way that it will become fold or highly condense to form chromosome.

Ans.5.cell lysis mean cell degrade by the enzyme which destroys its outer layer.i.e by lysozyme.

Ans.6.alcohol is on the top of the dna solution due to its non mixing because alcohol is of polar nature and dna solution is of non polar nature.

Ans.7.it is important not to mix alcohol in dna solution because it will seperate the dna and to become settle down in the solution

Ans.8.dna property is of presence of hydrogen bonds and by mixing of alcohol into them dehydration occur by which dna seperate from the solution by sticking on the glass rod is called dna spooling.

Ans.10.23 pairs chromosome in human