Question

12a. If you plated transformed cells on LB, what would the growth look like? Why?

b. How else could growth occur on plates with both antibiotics with regard to “successful” transformation? (Hint: How else could you both genes “get into” bacterial cells?)

c. What other ways can growth occur on plates with both antibiotics () that do not have anything to do with transformation/molecular cloning?

Answer 1

If you plated transformed cells on LB, what would the growth look like? Why?

Bacteria will grow on LB plate. Both transformed and non-transformed bacterial cells will grow on LB plate without having antibiotics. The colony will look likes white colony (it depends though). You can not differentiate transformed and non-transformed cells with only having LB on plate.

How else could growth occur on plates with both antibiotics with regard to “successful” transformation?

If both genes are incorporated (in transformed cells) and both genes represent antibiotics resistance, then the successful transformed cells can only grow on plate in presence of both antibiotics. Non-transformed cells can not grow. Transformed cells will contain both antibiotic resistance genes.

What other ways can growth occur on plates with both antibiotics that do not have anything to do with transformation/molecular cloning?

If the antibiotics are irrelevant with the transformation process then bacterial cells will not grow in presence of both antibiotics. The transformed bacteria carry certain antibiotic resistance gene (as for example kannamycine and ampicillin) but if you use other antibiotic that is not related to the experiment as for example tetracyclin then bacteria will not grow on plate because they dont have this certain antibiotic resistance gene.