Question

Have a look at the figure and answer the following questions (Note: LB is a standard growth medium like SOC).

1. What antibiotic are cells with 'Plasmid A' resistant to?12] 2. What antibiotic are cells with plasmid B, resistant to?[2] 3. Explain how 'Plasmid C' might allow bacteria with that plasmid to survive on plates containing ampicillin and kanamycin (i.e. what kind of genes do you think Plasmid C contains?).12) 4. What can we assume about Plasmid D'? (i.e. what kind of genes do you think Plasmid D does not contain?) [2

Answer 1

Answer:

Plasmid A is resistant to kanamycin antibiotic.

Plasmid B is resistant to ampicillin antibiotic.

Plasmid C is resistant to both ampicillin and kanamycin because it contains antibiotic resistant gene.

Plasmid D is not resistant to amphicilla and kanamycin because it not contain the antibiotic resistant gene.