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Name the salt which you have used in "salting out" experiment?
5. A. Name the salt which you have point) B. what is the purpose of salting -out? (1 point) 6. A. What is the difference be
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Answer #1

QUESTION 5

A)In this type of experiments, the most used salt is ammonium sulfate. However, depending of what you want to precipitate, you can vary the concentration of the salt. If you want to precipitate nucleic acids, you can use a concentration of 30% w/v of salt. If you want to precipitate protein, you can use 65% w/v.

B) The salt is used to reduce the solubility of the sample that you want to precipitate. In this context, the water molecules will attach to the ions of the salt, decreasing the water molecules attached to the protein and allowing its precipitation.

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