Hierarchical sequencing –
Requires the genome to be fragmented
Involves construction of a genomic library
The sequence of entire clone is determined.
The order of library clones is identified
Shotgun sequencing –
Requires the genome to be fragmented.
Only the sequences at the ends of clones are determined.
Adjacent sequences are assembled into long contigs based on overlap.
Classify each statement on the left to indicate if it is part of hierarchical or shotgun...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...