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Bb Problems, Set 6 Name A cross between an Hfr bacterial strain with genotype val met...
I'm not sure how to do part B, please help, and show the work if you...
I'm not sure how to do part B, please help, and show the work
if you can!
6-3 A cross between an Hfr bacterial strain with genotype val" met" tyr is crossed with an F strain with genotype val met" tyr". Val is known to enter last, and so val" recombinants are selected on medium that contains no val (only met and tyr). Recombinant colonies are then tested for met* and tyr*. The table summarizes the results. 0.8% O.1 -...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
2. A cross is made between two . coli strains: Hfr arg+ bio+leu+ X F arg-bio-leu-....
2. A cross is made between two . coli strains: Hfr arg+ bio+leu+ X F arg-bio-leu-. Interrupted mating studies show that arg+ enters the recipient last, and so arg+ recombinants are selected on a medium containing bio and leu only. These recombinants are tested for the presence of bio+ and leu+. The following numbers of individuals are found for each genotype: arg+ bio+ leu+ 320 argt bio- leu+ 0 arg+ bio+ leu-8 arg+ bio- leu 48 a. What is the...
Please answer all the questions. No explanation needed. 6. (pr) If a met-thr-Str. Hfr strain is...
Please answer all the questions. No explanation needed.
6. (pr) If a met-thr-Str. Hfr strain is mated with an F- of genotype lew-thi-Str prototrophic recombinants may be detected by plating the mixture on: A. Leucine and methionine + Streptomycin B. Threonine and thiamine + Streptomycin C. Leucine and thiamine and Streptomycin D. Minimal Medium + Streptomycin E. Methionine, threonine, leucine, and thiamine + Streptomycin 7. ( ) From an F+ strain with markers edebin that circular order), which of the...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-)...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-) and another yeast strain, which is His+Met+. One hundred asci were dissected, and colonies were grown from the four spores in each ascus. Cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium(min), min+histidine (his), min+methionine (met),or min+his+met. The number of tetrads with four spores that can grow in different type of media is shown in...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM 5cM 40cM If a diploid female heterozygous for A and D (A D//a d) is testcrossed to a homozygous recessive male (a d/a d), what percent of the progeny are predicted to have the genotype A d/a d? A. 100 B. 75 C. 50 D. 25 E. Can’t be determined 2. Yellow body (y), vermillion eye color (v) and miniature body (m) are recessive...
I'm not sure how to do part B, please help, and show the work
if you can!
6-3 A cross between an Hfr bacterial strain with genotype val" met" tyr is crossed with an F strain with genotype val met" tyr". Val is known to enter last, and so val" recombinants are selected on medium that contains no val (only met and tyr). Recombinant colonies are then tested for met* and tyr*. The table summarizes the results. 0.8% O.1 -...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
2. A cross is made between two . coli strains: Hfr arg+ bio+leu+ X F arg-bio-leu-. Interrupted mating studies show that arg+ enters the recipient last, and so arg+ recombinants are selected on a medium containing bio and leu only. These recombinants are tested for the presence of bio+ and leu+. The following numbers of individuals are found for each genotype: arg+ bio+ leu+ 320 argt bio- leu+ 0 arg+ bio+ leu-8 arg+ bio- leu 48 a. What is the...
Please answer all the questions. No explanation needed.
6. (pr) If a met-thr-Str. Hfr strain is mated with an F- of genotype lew-thi-Str prototrophic recombinants may be detected by plating the mixture on: A. Leucine and methionine + Streptomycin B. Threonine and thiamine + Streptomycin C. Leucine and thiamine and Streptomycin D. Minimal Medium + Streptomycin E. Methionine, threonine, leucine, and thiamine + Streptomycin 7. ( ) From an F+ strain with markers edebin that circular order), which of the...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-) and another yeast strain, which is His+Met+. One hundred asci were dissected, and colonies were grown from the four spores in each ascus. Cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium(min), min+histidine (his), min+methionine (met),or min+his+met. The number of tetrads with four spores that can grow in different type of media is shown in...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
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