Homework Answers
2. (a) Gene order is "arg bio leu".
(b) Map distance between arg and bio = 12.76%.
Map distance between bio and leu = 2.12%.
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2. A cross is made between two . coli strains: Hfr arg+ bio+leu+ X F arg-bio-leu-....
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. Across is made between an Hfr strain of the genotype StrSx-y+z-and an F-strain of the genotype StrRx+y-z+. Interrupte...
3. Across is made between an Hfr strain of the genotype StrSx-y+z-and an F-strain of the genotype StrRx+y-z+. Interrupted-mating studies show that of the three markers y+ is transferred least frequently to the exconjugants, and that the Str locus never enters the recipient strain. Selection for the y+ recombinants was followed by replica plating for the x and z alleles. The following data were obtained: x+ y+ z+ 79 x+ y+ z- 13 x- y+ z+ 4 x- y+ z-...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Genetics Question: I am confused on the second part of the question- determining the information for...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM...
1. The following diagram shows the genetic map for a chromosome. A B C D 10cM 5cM 40cM If a diploid female heterozygous for A and D (A D//a d) is testcrossed to a homozygous recessive male (a d/a d), what percent of the progeny are predicted to have the genotype A d/a d? A. 100 B. 75 C. 50 D. 25 E. Can’t be determined 2. Yellow body (y), vermillion eye color (v) and miniature body (m) are recessive...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Genetics Question: I am confused on the second part of the
question- determining the information for both the donor and
recipient. Thank you!
different interrupted mating experiments, you obtain the following data for 2. Ih three the time of entry of the following genes: arg, met, leu, ser, gal, val, and phe. All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli. Experiment 1 (Hfr 1)...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
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