3. Across is made between an Hfr strain of the genotype StrSx-y+z-and an F-strain of the genotype StrRx+y-z+. Interrupte...
3. Across is made between an Hfr strain of the genotype StrSx-y+z-and an F-strain of the genotype StrRx+y-z+. Interrupted-mating studies show that of the three markers y+ is transferred least frequently to the exconjugants, and that the Str locus never enters the recipient strain. Selection for the y+ recombinants was followed by replica plating for the x and z alleles. The following data were obtained:
x+ y+ z+ 79
x+ y+ z- 13
x- y+ z+ 4
x- y+ z- 254
Total 350
a.What is the correct gene order in relationship to the origin of transfer?
b. Calculate the distance between each pair of genes. Show your work.
Homework Answers
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3. Across is made between an Hfr strain of the genotype StrSx-y+z-and an F-strain of the genotype StrRx+y-z+. Interrupte...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In separate experiments using the technique of interrupted mating, these four Hfr strains donated the following markers to an auxotrophic, str F strain in the order shown: (only the first six genes transmitted were scored for each strain) Hfr Strain 1 Hfr Strain 2 Hfr Strain 3 Hfr Strain 4 A. What is the order of these markers on the circular chromosome of the original...
2. A cross is made between two . coli strains: Hfr arg+ bio+leu+ X F arg-bio-leu-....
2. A cross is made between two . coli strains: Hfr arg+ bio+leu+ X F arg-bio-leu-. Interrupted mating studies show that arg+ enters the recipient last, and so arg+ recombinants are selected on a medium containing bio and leu only. These recombinants are tested for the presence of bio+ and leu+. The following numbers of individuals are found for each genotype: arg+ bio+ leu+ 320 argt bio- leu+ 0 arg+ bio+ leu-8 arg+ bio- leu 48 a. What is the...
7. Five Hfr strains A through E are derived from a single F strain of E....
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str S i+...
4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str S i+ o+ z+ y+ (that you grew in the absence of lactose) with a lac - F - strain with the genotype str R i- o+ z - y. You allow sufficient time for the transfer of the genes indicated, with the exception of str S , and plate the bacteria on minimal medium supplemented with arabinose and streptomycin but no lactose. You observe transient...
Bb Problems, Set 6 Name A cross between an Hfr bacterial strain with genotype val met...
Bb Problems, Set 6 Name A cross between an Hfr bacterial strain with genotype val met tyr.is crossed with an F strain with genotype val met tyr. Val is known to enter last, and so val" recombinants are selected on medium that contains no val (only met and tyr). Recombinant colonies are then tested for met and tyr". The table summarizes the results. #Colonies 552 valt met tyrt Genotype valt met+tyrt valt met- tyr+ valt met- tyr. valt met+ tyr-...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
This is a Hfr mapping question and I'm completely lost here Basically the bonus is a...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
Four Hfr strains (1, 2, 3, 4) were isolated from the same prototrophic, strF strain. In separate experiments using the technique of interrupted mating, these four Hfr strains donated the following markers to an auxotrophic, str F strain in the order shown: (only the first six genes transmitted were scored for each strain) Hfr Strain 1 Hfr Strain 2 Hfr Strain 3 Hfr Strain 4 A. What is the order of these markers on the circular chromosome of the original...
2. A cross is made between two . coli strains: Hfr arg+ bio+leu+ X F arg-bio-leu-. Interrupted mating studies show that arg+ enters the recipient last, and so arg+ recombinants are selected on a medium containing bio and leu only. These recombinants are tested for the presence of bio+ and leu+. The following numbers of individuals are found for each genotype: arg+ bio+ leu+ 320 argt bio- leu+ 0 arg+ bio+ leu-8 arg+ bio- leu 48 a. What is the...
7. Five Hfr strains A through E are derived from a single F strain of E. coli. The following chart shows the entry times in minutes of the first five markers into an F strain when each Hfr strain is used in an interrupted-conjugation experiment: A B C E pro gal mal strs ser ade his ade This gal pro mer 11 16 36 51 13 28 38 44 70 prot met xyl mal" strs 29 32 37 his 10...
Bb Problems, Set 6 Name A cross between an Hfr bacterial strain with genotype val met tyr.is crossed with an F strain with genotype val met tyr. Val is known to enter last, and so val" recombinants are selected on medium that contains no val (only met and tyr). Recombinant colonies are then tested for met and tyr". The table summarizes the results. #Colonies 552 valt met tyrt Genotype valt met+tyrt valt met- tyr+ valt met- tyr. valt met+ tyr-...
3. (10 pts) A friend sets up the following cross between an Hfr strain and an F- strain; Hfr: rif lac str pheA X F:rif lac str pheA In the Hfr, the F factor is integrated at 9 minutes on the E. coli genetic map and transfers the genome in a counter clockwise direction, similar to CSHI19 Gene function Position on genetic map Gene Name RNA polymerase (target of rifamycin) lactose utilization phenylalanine biosynthesis ribosomal protein (target of streptomycin) rpoB...
This is a Hfr mapping question and I'm completely lost
here
Basically the bonus is a puzzle. It is based on mapping done with 4 different Hfr strains. I've given you the minutes that that it takes for several of the markers on the map to transfer in as determined by Hfr mapping. All you have to do is put them in the correct order around the circular chromosome and show me how many minutes are in between each marker...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
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