Question

This PCR step is called annealing. The annealing step follows the denaturation step: it is usually the lowest temperature in the PCR. The temperature of this step varies with each PCR reaction because each primer has its own sequence and may not be an identical match to the DNA template strand. GC or CG have three hydrogen bonds and AT or TA have two hydrogen bonds. Higher annealing temperatures are more stringent and require a better match between primer and template. C. Build two new DNA strands as follows. At the 3' side of the primer, attach a nucleotide base puzzle piece to the primer while making sure that the nucleotide base complements the large DNA strand where the primer has annealed. In the PCR. this is called synthesis or extension. It is called synthesis because the enzyme you added to the PCR called (Fill in the blank) is creating the complementary DNA strand, and extension because the 5' to 3' DNA strand is being elongated (extended) at the 3'terminus. The temperature of this step is the optimal temperature for the synthesis enzyme Taq polymerase, usually 72°C. 1. Write the sequence of the DNA strands using colored pencils as noted to fill in the boxes below. a. Use black ink/pencil for strand you created that is complementary to the template sequence. b. Use dark blue pencil to write in the left primer sequence in the appropriate location. c. Use light blue pencil for the newly synthesized strand of DNA extended at the left primer site. d. Use dark green pencil to write in the right primer sequence in the appropriate location. e. Use light green for the newly synthesized strand of DNA extended at the right primer site. f. Circle the bases in orange pencil on both long strands that were not copied in the PCR. These bases represent the remainder of the DNA strand that made up the original chromosome in the DNA template you extracted/isolated. Tested Studies for Laboratory Teaching

Answer 1

Polymerase (fill in the blanks)

5' ATCG CACCGG TAATGAG CITCAT|3' IA GEGIGGELAII G'ACTć 65' right primer TAG| сте сс ATTA cТceATA 57 5'CACC GG I TA ATGAG CTCAT 6 left priner

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