Lac I ( Gene)- non functional cannot bind the inducer How does it affect in the...
Lac I ( Gene)- non functional cannot bind the inducer
How does it affect in the presence of lactose?
How does it affect in absence of lactose?
Lac Z( protein)- Wipeout mutation
How does it affect in the presence of lactose?
How does it affect in absence of lactose?
Homework Answers
If lac I gene of lac operon is non functional and cannot bind the inducer, all three structural genes of lac operon are transcribed regardless of the presence or absence of inducer.
Explanation: If lac I gene of lac operon is functional and inducer is present, the product of lac I gene (repressor) binds with the inducer and gets inactivated. Inactive repressor allows transcription of structural genes of lac operon. If lac I gene of lac operon is functional and inducer is absent, the product of lac I gene (repressor) does not binds with the inducer and remains active. Active repressor prevents transcription of structural genes of lac operon.But, the product of lac I gene ( repressor) is not available if lac I gene is non functional. As a result, transcription of structural genes of lac operon continues regardless of of presence or absence of inducer.
In wipeout mutation of lac Z (protein), there is production of lac Y(protein) and lac A (protein) in the presence of inducer. In other words, lac operon is induced in the presence of inducer. Lac operon is not induced in the absence of inducer.
Add Answer to:
Lac I ( Gene)- non functional cannot bind the
inducer
How does it affect in the...
If I mutate Lac I (find out what Lac I makes) - if I make it...
If I mutate Lac I (find out what Lac I makes) - if I make it non-functional meaning it can not bind the inducer. Mutate Lac I, can't bind to the inducer, what happens to the operon in the presence of lactose and absence of lactose? • Mutate Lac Z gene - wipeout mutation, meaning you cannot transcribe the gene! What happens to the presence of lactose and in the absence of lactose?
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is...
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is absent. Predict the ways in which the following conditions will affect the transcription of the lactose-utilization genes. OPERON Regulatory Promoter Operator_ gene Lactose-utilization genes DNA mRNA RNA polymerase cannot attach to promoter Active repressor Protein If a mutation in the regulatory gene results in a misfolding of the repressor protein so that it can no longer bind DNA, the lactose-utilization genes O Will be...
(3 pts) There is a new mutation in the Lac/gene, called IN, that introduces a missense...
(3 pts) There is a new mutation in the Lac/gene, called IN, that introduces a missense mutation in the DNA binding domain of the protein. IN can not bind to the operator. Predict the outcome of the following lac operon genotype for the expression of functional lac enzymes in the different conditions indicated. (Note: + = wildtype, - = no functional protein made.) IN O+P+ Z-Y+ - lactose + lactose b-gal (Z) Permease (Y) b-gal (Z) Permease (Y) + +...
For each genotype, with and without inducer, select whether or not a functional galactosidase or permease...
For each genotype, with and without inducer, select whether or not a functional galactosidase or permease will be present. IS indicates a super-repressor (it can’t bind to lactose/inducers). OC indicates a constitutive operon (no repressor can bind to this region of DNA). I- ; Z- ; Y- each indicate an allele that does not produce a functional protein. P- indicates a mutation in the promoter region such that RNA polymerase does not bind. Group of answer choices I+ P+ O+...
1- For each genotype, with and without inducer, select whether or not a functional galactosidase ...
1- For each genotype, with and without inducer, select whether or not a functional galactosidase or permease will be present. IS indicates a super-repressor (it can’t bind to lactose/inducers). OC indicates a constitutive operon (no repressor can bind to this region of DNA). I- ; Z- ; Y- each indicate an allele that does not produce a functional protein. P- indicates a mutation in the promoter region such that RNA polymerase does not bind. ----------------------------------------------------------------------------------------------------------------- I+ P+ OC Z+ Y+...
A. Describe in detail the regulation of the prokaryotic lac operon for the following environment: Glucose...
A. Describe in detail the regulation of the prokaryotic lac operon for the following environment: Glucose is present, lactose present, & the repressor protein is mutated such that the allosteric site is non-functional and can no longer bind its substrate. Be sure to specify the presence or absence and location of Lac I, allolactose, cAMP, CAP, and RNA polymerase. B. Indicate whether transcription is occurring at a basal level, a high level, or not at all.
Lac Operon Homework . Unanswered How would a mutation in the lacy gene effect the lac...
Lac Operon Homework . Unanswered How would a mutation in the lacy gene effect the lac operon o A There would be normal levels of lac operon expression O B There would be over expression of the lac operon because it would not be repressible O C There would be under expression of the lac operon because of the absence of the inducer Unanswered
Most of what we know about the lac operon in E. coli has come from the...
Most of what we know about the lac operon in E. coli has come from the genetic analysis of various mutants. Below is a list of mutants for regions of the operon. A + superscript indicates no mutation and normal function of that region, - indicates a knock out and no function of that region, c indicates the mutation resulted in constitutive action of that region. The effect of the mutation is determined by expression of the lacZ gene as...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions,...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
If I mutate Lac I (find out what Lac I makes) - if I make it non-functional meaning it can not bind the inducer. Mutate Lac I, can't bind to the inducer, what happens to the operon in the presence of lactose and absence of lactose? • Mutate Lac Z gene - wipeout mutation, meaning you cannot transcribe the gene! What happens to the presence of lactose and in the absence of lactose?
The diagram below illustrates the LAC operon in its OFF state when the inducer molecule —lactose—is absent. Predict the ways in which the following conditions will affect the transcription of the lactose-utilization genes. OPERON Regulatory Promoter Operator_ gene Lactose-utilization genes DNA mRNA RNA polymerase cannot attach to promoter Active repressor Protein If a mutation in the regulatory gene results in a misfolding of the repressor protein so that it can no longer bind DNA, the lactose-utilization genes O Will be...
(3 pts) There is a new mutation in the Lac/gene, called IN, that introduces a missense mutation in the DNA binding domain of the protein. IN can not bind to the operator. Predict the outcome of the following lac operon genotype for the expression of functional lac enzymes in the different conditions indicated. (Note: + = wildtype, - = no functional protein made.) IN O+P+ Z-Y+ - lactose + lactose b-gal (Z) Permease (Y) b-gal (Z) Permease (Y) + +...
Lac Operon Homework . Unanswered How would a mutation in the lacy gene effect the lac operon o A There would be normal levels of lac operon expression O B There would be over expression of the lac operon because it would not be repressible O C There would be under expression of the lac operon because of the absence of the inducer Unanswered
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
Most questions answered within 3 hours.
-
(Expected rate of return and risk) Carter Inc. is evaluating a
security. Calculate the investment’s expected...
asked 2 hours ago -
What specific indicators can point to lack of progress for
African Americans in American society?
asked 3 hours ago -
1-The Electrons in a beam are moving at 2.7×108 m/s in an
electric field of 15000...
asked 3 hours ago -
A gas tank is a vertical cylinder. It has a radius of 1m, a
height of...
asked 3 hours ago -
Accent Software faces the following conditions. All of these
support Accent’s use of a market-penetration pricing...
asked 4 hours ago -
A mathematically inclined friend emails you the following
instructions: "Meet me in the cafeteria the first...
asked 4 hours ago -
A monopoly sells in two countries . The demand curves in the two
countries are p1...
asked 5 hours ago -
A .15kg rubber ball is bounced off a wall. Before hitting the
wall, the ball moves...
asked 6 hours ago -
A manufacturing company preparing to build a new plant is
considering three potential locations for it....
asked 6 hours ago -
B. If compound Y has approximately the same values of solubility
in toluene as compound X,...
asked 7 hours ago -
Oscar Inc. has inventory in Japan valued at 39,051,000 Yen one
year ago. One year ago...
asked 7 hours ago -
If Canada suffered from "fundamental disequilibrium," and its
government choose not to devalue its currency, a...
asked 7 hours ago